Pathogenic ‘Bison-type’ Mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo ( Bubalus bubalis ) in North India

Abstract Despite low per-animal productivity of ruminants in developing countries, Johne's disease has not been investigated in buffaloes, which are primarily found in these countries. This is due to lack of expertise, diagnostic kits and priority to production diseases like Johne's diseas...

Full description

Saved in:
Bibliographic Details
Published in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2008-07, Vol.31 (4), p.373-387
Main Authors: Yadav, D, Singh, S.V, Singh, A.V, Sevilla, I, Juste, R.A, Singh, P.K, Sohal, J.S
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Bacteriology
Biological and medical sciences
Bison
Bubalus bubalis
Buffaloes
Buffaloes - microbiology
Culture de Tissus
ELISA kit
EST 1311 PCR–REA
EST 900 PCR–REA
Fundamental and applied biological sciences. Psychology
Genotype
India - epidemiology
Infectious Disease
IS 1311 PCR–REA
IS 900 PCR–REA
Johne's disease
Kit d'ELISA
La maladie de Johne
Microbiology
Miscellaneous
Mycobacterium avium
Mycobacterium avium paratuberculose de sous-espèces
Mycobacterium avium subsp. paratuberculosis - classification
Mycobacterium avium subsp. paratuberculosis - genetics
Mycobacterium avium subsp. paratuberculosis - isolation & purification
Mycobacterium avium subspecies paratuberculosis
Paratuberculosis - diagnosis
Paratuberculosis - epidemiology
Paratuberculosis - microbiology
Pilot Projects
Prevalence
Prédominance
Ruminantia
Tissues culture
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Despite low per-animal productivity of ruminants in developing countries, Johne's disease has not been investigated in buffaloes, which are primarily found in these countries. This is due to lack of expertise, diagnostic kits and priority to production diseases like Johne's disease. Presence of pathogenic Mycobacterium avium subspecies paratuberculosis (Map) was investigated by screening of target tissues (mesenteric lymph nodes and large intestine) by culture and IS 900 PCR, in 50 sacrificed buffaloes. Indigenous ELISA kit originally developed for goats and sheep was standardized in buffaloes and used to estimate sero-presence of Map in 167 serum samples representing population of buffaloes in Agra region of North India. In culture, 48.0% buffaloes were positive from 50 tissues each from mesenteric lymph nodes (34.0%) and large intestine (36.0%). IS 900 PCR was standardized using specific primers (150 C and 921) and 229 bp-amplified product was characteristic for Map. Of the 25 mesenteric lymph nodes, 40.0% were positive in IS 900 PCR. Genomic DNA from Map cultures was successfully amplified from all the 24 isolates (100.0%). Map was further genotyped as ‘Bison type’ using IS 1311 PCR–REA. Culture of tissues showed high presence of Map in target tissues, despite high culling rate in buffalos in view of high demand of buffalo meat. Specific tissue-PCR provided rapid confirmation of Map infection in sacrificed buffaloes. In tissue-PCR, all the cultures were positive as compared to 40.0% detected directly from tissues. ELISA kit using indigenous protoplasmic antigen was highly sensitive as compared to commercial antigen in detecting Map infection therefore, could be used as ‘Herd Screening Test’ in buffaloes against Johne's disease. This pilot study first time reports a highly pathogenic ‘Bison-type’ genotype of M. avium subspecies paratuberculosis from the riverine buffaloes ( Bubalus bubalis ) of Agra region in North India.
ISSN:0147-9571
1878-1667
DOI:10.1016/j.cimid.2007.06.007