Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors

Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Frequencies of T cells producing cytokines after stimulation by CMV Agweremeasured in hematopoietic stem-cell donors using flow cytometry. In seropositive individuals (n = 75) the mean...

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Bibliographic Details
Published in:Cytotherapy (Oxford, England) England), 2002-01, Vol.4 (1), p.29-40
Main Authors: Hensel, N., Melenhorst, J.J., Bradstock, K., Schwarer, A.P., Eniafe, R., Nakamura, R., Barrett, A.J.
Format: Article
Language:English
Subjects:
Adoptive Transfer - methods
Antigens, Viral - immunology
CMV
Cytomegalovirus
Cytomegalovirus - immunology
Flow Cytometry - methods
Hematopoietic Stem Cell Transplantation
Humans
Immunologic Memory
Interferon-gamma - biosynthesis
interferon-γ
intracellular cytokine
lymphocyte
T-Lymphocyte Subsets - classification
T-Lymphocyte Subsets - immunology
Tissue Donors
TNF-α
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Frequencies of T cells producing cytokines after stimulation by CMV Agweremeasured in hematopoietic stem-cell donors using flow cytometry. In seropositive individuals (n = 75) the mean number of CD81 (CD8bright, CD8dim) and CD4+ cells producing IFN-γ was respectively 3.1% (12.6/μL) and 0.38% (3.2/μL), over 10–fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in both CD4+ and CD8+ cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD41 cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency. These quantitative studies and the recognition of CD4+ cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation.
ISSN:1465-3249
1477-2566
DOI:10.1080/146532402317251509