Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents

With the incorporation of lysozyme during the immobilization step, considerable enhancement of the operational stability of a biosensor has been demonstrated in the case of an immobilized single enzyme (glucose oxidase) system for glucose and multienzyme (invertase, mutarotase and glucose oxidase) s...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2002-06, Vol.17 (6), p.503-507
Main Authors: Gouda, M.D., Kumar, M.A., Thakur, M.S., Karanth, N.G.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensor
Biosensors
Biotechnology
Cattle
Detachable membrane unit
Enzyme Stability
Enzymes, Immobilized
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Gelatin - chemistry
Glucose - analysis
Glucose - chemistry
Immobilized enzymes
Methods. Procedures. Technologies
Muramidase - chemistry
Operational stability
Protein based stabilizing agents
Quality Control
Reproducibility of Results
Serum Albumin, Bovine - chemistry
Sucrose - analysis
Sucrose - chemistry
Various methods and equipments
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Summary:With the incorporation of lysozyme during the immobilization step, considerable enhancement of the operational stability of a biosensor has been demonstrated in the case of an immobilized single enzyme (glucose oxidase) system for glucose and multienzyme (invertase, mutarotase and glucose oxidase) system for sucrose. Thus an increased number of repeated analyses of 750 samples during 230 days for glucose and 400 samples during 40 days of operation for sucrose have been achieved. The increased operational stability of immobilized single and multienzyme system, will improve the operating cost effectiveness of the biosensor.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(02)00021-0