Filamentous fungi as cell factories for heterologous protein production

Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools hav...

Full description

Saved in:
Bibliographic Details
Published in:Trends in Biotechnology 2002-05, Vol.20 (5), p.200-206
Main Authors: Punt, Peter J, van Biezen, Nick, Conesa, Ana, Albers, Alwin, Mangnus, Jeroen, van den Hondel, Cees
Format: Article
Language:English
Subjects:
Aspergillus
Biological and medical sciences
Biotechnology
Cloning
controlled fermentation
cytokines
Enzymes
Factories
Fermentation - genetics
Fermentation - physiology
Food
Fundamental and applied biological sciences. Psychology
Fungi
Fungi - genetics
Fungi - metabolism
gene technology
Genes
Genetic engineering
Genetic technics
Genomes
GRAS status
Humans
industrial enzymes
Interleukin-6 - analysis
Interleukin-6 - biosynthesis
Interleukin-6 - genetics
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microorganisms
Modification of gene expression level
peroxidases
Peroxidases - analysis
Peroxidases - biosynthesis
Peroxidases - genetics
Protein Conformation
protein secretion
Proteins
Recombinant Proteins - analysis
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Technological change
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the ‘genomics’ technology will further develop high-throughput technologies for these organisms. Filamentous fungi have become preferred host organisms for protein production through a combination of molecular tools and process development.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(02)01933-9