Tazarotene-Induced Gene 1 (TIG1) Expression in Prostate Carcinomas and Its Relationship to Tumorigenicity

Background: Prostate cancer is the most common noncutaneous male cancer and one of the least understood malignant diseases. Identifying key genetic factors involved in the metastasis of prostate cancer cells is critical. In this study, we used selective subtractive differential gene display to ident...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 2002-04, Vol.94 (7), p.482-490
Main Authors: Jing, Chun, El-Ghany, Manal Abd, Beesley, Carol, Foster, Christopher S., Rudland, Philip S., Smith, Paul, Ke, Youqiang
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
In Situ Hybridization
Male
Medical sciences
Membrane Proteins
Mice
Mice, Inbred BALB C
Mice, Nude
Molecular Sequence Data
Nephrology. Urinary tract diseases
Nicotinic Acids - genetics
Nicotinic Acids - metabolism
Prostatic Hyperplasia - genetics
Prostatic Hyperplasia - metabolism
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Proteins - genetics
Retinoids - genetics
Retinoids - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Transfection
Tumor Cells, Cultured
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:Background: Prostate cancer is the most common noncutaneous male cancer and one of the least understood malignant diseases. Identifying key genetic factors involved in the metastasis of prostate cancer cells is critical. In this study, we used selective subtractive differential gene display to identify a gene whose decreased expression may contribute to the growth and expansion of prostate cancer. Methods: We used 192 primer pair combinations and polymerase chain reaction technology to identify genes expressed in the benign prostate cell line PNT-2 but not in the malignant prostate cancer cell lines LNCaP, Du-145, PC-3, or PC-3M. The tazarotene-induced gene 1 (TIG1) was chosen for further study. TIG1 expression in normal tissues and cell lines was analyzed by northern blot and in normal and tumor prostate tissue sections by in situ hybridization. The in vitro invasiveness (migration through extracellular matrix) and in vivo tumorigenicity (growth in nude mice) were assessed for the highly malignant PC-3M cell line transfected with TIG1 or control cDNA. All statistical tests were two-sided. Results: TIG1 mRNA was expressed in a variety of normal tissues other than prostate tissue. TIG1 mRNA was detected in all 10 normal human prostate tissues and all 51 benign prostatic hyperplastic tissues analyzed but in only four of 51 malignant prostate tissues analyzed. Compared with vector-transfected cells, transfection of PC-3M cells with TIG1 decreased in vitro invasiveness from 14.7% to 3.7%, (mean difference = 11%; 95% confidence interval [CI] = 9.2% to 12.8%, P
ISSN:0027-8874
1460-2105
1460-2105
DOI:10.1093/jnci/94.7.482