Transcription of the M1 muscarinic receptor gene in neurons and neuronal progenitors of the embryonic rat forebrain

Development of the nervous system is accompanied by expansion and differentiation of the neuronal progenitors within the embryonic neuroepithelium. Although the role of growth factors in this process is well documented, there is increasing evidence for a role of neurotransmitters. Acetylcholine is k...

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Bibliographic Details
Published in:Journal of neurochemistry 2004-01, Vol.88 (1), p.70-77
Main Authors: Williams, Brenda P., Milligan, Carol J., Street, Miyoko
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Differentiation - physiology
Cells, Cultured
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Epithelial Cells - cytology
Epithelial Cells - metabolism
Exons
Fundamental and applied biological sciences. Psychology
Genes, Reporter
muscarinic receptor
neural stem cells
neuronal progenitor cells
Neurons - cytology
Neurons - metabolism
Prosencephalon - cytology
Prosencephalon - embryology
Prosencephalon - metabolism
Rats
Rats, Sprague-Dawley
Receptor, Muscarinic M1 - biosynthesis
Receptor, Muscarinic M1 - genetics
Regulatory Sequences, Nucleic Acid - physiology
Stem Cells - cytology
Stem Cells - metabolism
Transcription, Genetic
Vertebrates: nervous system and sense organs
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Summary:Development of the nervous system is accompanied by expansion and differentiation of the neuronal progenitors within the embryonic neuroepithelium. Although the role of growth factors in this process is well documented, there is increasing evidence for a role of neurotransmitters. Acetylcholine is known to exert many actions on developing neural cells, but its potential role in neurogenesis is unclear. Here, we show that the M1 muscarinic acetylcholine receptor is expressed in the neuroepithelium of the rat forebrain, where it is found on both nestin+ progenitor cells and TuJ1+ newly differentiated neurons. Furthermore, transcription is governed, at least in part, by regulatory cis elements that are also responsible for driving transcription in neuroblastoma cells. This represents the first demonstration of M1 receptors on neuronal progenitor cells and supports the notion that M1 muscarinic receptors may play a role in development of the nervous system prior to the onset of synaptogenesis and their subsequent role in neurotransmission.
ISSN:0022-3042
1471-4159
DOI:10.1111/j.1471-4159.2004.02117.x