Molecular characterization of nucleus-localized RNA-binding proteins from higher plants

We report the isolation and characterization of genes from the higher plants Arabidopsis, spinach and tobacco which code for nucleus-localized RNA-binding proteins. Common features of these polypeptides are glycine/arginine-rich regions with several RGG repeats at their N- and C-termini, which are s...

Full description

Saved in:
Bibliographic Details
Published in:Plant molecular biology 2002-03, Vol.48 (4), p.413-421
Main Authors: Landsberger, Martin, Lorković, Zdravko J, Oelmüller, Ralf
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Cell Nucleus - metabolism
DNA, Complementary - genetics
DNA, Plant - genetics
Genes
Glucuronidase - genetics
Glucuronidase - metabolism
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana tabacum
NtRGG1 protein
Nuclear Localization Signals - genetics
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Plants - genetics
Plants - metabolism
Polypeptides
Protein Binding
Proteins
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Repetitive Sequences, Amino Acid - genetics
RNA - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Spinacea oleracea
Spinacia oleracea - genetics
Spinacia oleracea - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report the isolation and characterization of genes from the higher plants Arabidopsis, spinach and tobacco which code for nucleus-localized RNA-binding proteins. Common features of these polypeptides are glycine/arginine-rich regions with several RGG repeats at their N- and C-termini, which are sufficient for RNA binding in northwestern assays. All polypeptides analysed contain two basic bipartite nuclear localization signals and translational fusions harbouring these regions with the beta-glucuronidase gene direct the fusion proteins into the nucleus. Nuclear localization was confirmed by cellular fractionation with a polyclonal antiserum raised against the over-expressed tobacco protein NtRGG1p. Two or three copies of related RGG genes appear to be present in the analysed organisms and the expression of some of them is regulated: a tobacco gene is light-regulated and a spinach gene is preferentially expressed in roots. Possible biological functions of this class of RNA-binding proteins as well as structure/function relationships related to the modular structure are discussed.
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1014089531125