Immunocompetent Infants as a Human Reservoir for Pneumocystis jirovecii: Rapid Screening by Non-Invasive Sampling and Real-Time PCR at the Mitochondrial Large Subunit rRNA Gene

We tested a real-time PCR assay targeting the Pneumocystis jirovecii mitochondrial large subunit rRNA gene on 240 archival nasopharyngeal aspirates from non-immunosuppressed infants. The sensitivity of this assay appeared close to that of a conventional nested-PCR assay targeting the same locus. Bec...

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Bibliographic Details
Published in:The Journal of eukaryotic microbiology 2003, Vol.50 (6), p.668-669
Main Authors: TOTET, ANNE, MELIANI, LEILA, LACUBE, PHILIPPE, PAUTARD, JEAN CLAUDE, RACCURT, CHRISTIAN, ROUX, PATRICIA, NEVEZ, GILLES
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Bronchiolitis - immunology
Bronchiolitis - microbiology
Disease Reservoirs
DNA Primers
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
DNA, Mitochondrial - genetics
DNA, Mitochondrial - isolation & purification
Gene Amplification
Genes, Reporter
Human mycoses
Humans
Immunocompetence - immunology
Infant
Infectious diseases
Medical sciences
Mycoses
Mycoses of the respiratory system
Pneumocystis carinii - genetics
Pneumocystis carinii - immunology
Pneumonia, Pneumocystis - immunology
Polymerase Chain Reaction - methods
RNA, Fungal - genetics
RNA, Ribosomal - genetics
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Summary:We tested a real-time PCR assay targeting the Pneumocystis jirovecii mitochondrial large subunit rRNA gene on 240 archival nasopharyngeal aspirates from non-immunosuppressed infants. The sensitivity of this assay appeared close to that of a conventional nested-PCR assay targeting the same locus. Because of its one-step procedure, and its sensitivity and rapidity, the real-time PCR assay is particularly suitable for screening individuals parasitized by P. jirovecii within large populations.
ISSN:1066-5234
1550-7408
DOI:10.1368/1066-5234(2003)050[0668:IIAAHR]2.0.CO;2