Effects of experimental lower-limb ischaemia-reperfusion injury on the mesenteric microcirculation

Background: Ischaemia–reperfusion (I‐R) of the leg is associated with functional and structural changes in the intestine. This study assessed whether acute hind‐limb I‐R in rats induced a reduction in perfusion and/or signs of an inflammatory response in the intestine. Methods: Rats were subjected t...

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Bibliographic Details
Published in:British journal of surgery 2002-02, Vol.89 (2), p.185-191
Main Authors: Wehrens, X. H. T., Rouwet, E. V., oude Egbrink, M. G. A., Slaaf, D. W., Ramsay, G.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood Flow Velocity - physiology
Fundamental and applied biological sciences. Psychology
Heart Rate - physiology
Hemodynamics. Rheology
Hindlimb - blood supply
Leukocytes - physiology
Male
Microcirculation - physiology
Rats
Rats, Inbred Lew
Reperfusion Injury - physiopathology
Splanchnic Circulation - physiology
Vertebrates: cardiovascular system
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Summary:Background: Ischaemia–reperfusion (I‐R) of the leg is associated with functional and structural changes in the intestine. This study assessed whether acute hind‐limb I‐R in rats induced a reduction in perfusion and/or signs of an inflammatory response in the intestine. Methods: Rats were subjected to 2 h of unilateral hind‐limb ischaemia followed by 2 h of reperfusion (I‐R group, n = 9) or to a sham procedure (control group, n = 9). Mesenteric microvascular diameters, red blood cell velocity, blood flow and leucocyte–vessel wall interactions during reperfusion were measured using intravital microscopy. Results: Blood pressure and heart rate decreased from 30 min of reperfusion onwards in the I‐R group compared with controls. From 15 min after the start of reperfusion, mesenteric arteriolar and venular red blood cell velocity and blood flow decreased by 40–50 per cent. Microvascular diameters and leucocyte–vessel wall interactions did not change. Conclusion: Restoration of blood flow to an acutely ischaemic hind limb led to a significant decline in the splanchnic microcirculatory blood flow. There were, however, no signs of an early inflammatory response in the gut. © 2002 British Journal of Surgery Society Ltd
ISSN:0007-1323
1365-2168
DOI:10.1046/j.1365-2168.2002.01973.x