Local and systemic wound‐induction of RNase and nuclease activities in Arabidopsis: RNS1 as a marker for a JA‐independent systemic signaling pathway

Summary Induction of defense‐related genes is one way in which plants respond to mechanical injury. We investigated whether RNases are involved in the wound response in Arabidopsis thaliana. As in other plant systems, several activities are induced with various timings in damaged leaves, stems and s...

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Published in:The Plant journal : for cell and molecular biology 2002-02, Vol.29 (4), p.393-403
Main Authors: LeBrasseur, Nicole D., MacIntosh, Gustavo C., Pérez‐Amador, Miguel A., Saitoh, Maki, Green, Pamela J.
Format: Article
Language:English
Subjects:
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis thaliana
Biological and medical sciences
Biomarkers
Cyclopentanes - pharmacology
Deoxyribonucleases - genetics
Deoxyribonucleases - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Plant - drug effects
Generalities. Disease free stocks
jasmonic acid
Metabolism
Metabolism. Physicochemical requirements
oligogalacturonides
oligosaccharides
Oligosaccharides - pharmacology
Oxylipins
Phytopathology. Animal pests. Plant and forest protection
Plant Leaves - enzymology
Plant Leaves - genetics
Plant physiology and development
ribonuclease
Ribonucleases - genetics
Ribonucleases - metabolism
RNA, Plant - metabolism
RNS1 gene
Signal Transduction
Stress, Mechanical
systemic signaling
wounding
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Summary:Summary Induction of defense‐related genes is one way in which plants respond to mechanical injury. We investigated whether RNases are involved in the wound response in Arabidopsis thaliana. As in other plant systems, several activities are induced with various timings in damaged leaves, stems and seedlings in Arabidopsis, including at least three bifunctional nucleases, capable of degrading both RNA and DNA, as well as RNS1, a member of the ubiquitous RNase T2 family of RNases. The strong induction of RNS1 is particularly interesting because it occurs both locally and systemically following wounding. The systemic induction of this RNase indicates that members of this family may be involved in defense mechanisms in addition to their previously hypothesized functions in nutrient recycling and remobilization. Additionally, the systemic induction appears to be controlled independently of jasmonic acid, and the local induction of RNS1 and the nuclease activities are independent of both JA and oligosaccharide elicitors. Consequently, a novel systemic pathway, likely involving a third signal, appears to exist in Arabidopsis.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313x.2002.01223.x