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stella Is a Maternal Effect Gene Required for Normal Early Development in Mice
stella is a novel gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells [1, 2]. It encodes a protein with a SAP-like domain [3] and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing....
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Published in: | Current biology 2003-12, Vol.13 (23), p.2110-2117 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | stella is a novel gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells [1, 2]. It encodes a protein with a SAP-like domain [3] and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Here, we have investigated the effects of a targeted mutation of stella in mice. We show that while matings between heterozygous animals resulted in the birth of apparently normal stella null offspring, stella-deficient females displayed severely reduced fertility due to a lack of maternally inherited Stella-protein in their oocytes. Indeed, we demonstrate that embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage. stella is thus one of few known mammalian maternal effect genes [4–9], as the phenotypic effect on embryonic development is mainly a consequence of the maternal stella mutant genotype. Furthermore, we show that STELLA that is expressed in human oocytes [10] is also expressed in human pluripotent cells and in germ cell tumors. Interestingly, human chromosome 12p, which harbours STELLA, is consistently overrepresented in these tumors [11]. These findings suggest a similar role for STELLA during early human development as in mice and a potential involvement in germ cell tumors. |
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ISSN: | 0960-9822 1879-0445 |
DOI: | 10.1016/j.cub.2003.11.026 |