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Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease
The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD. We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5...
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| Published in: | Stroke (1970) 2003-12, Vol.34 (12), p.2835-2841 |
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| container_end_page | 2841 |
| container_issue | 12 |
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| container_title | Stroke (1970) |
| container_volume | 34 |
| creator | KIM, Seung-Ki YOO, Jong-Il CHO, Byung-Kyu SOO JIN HONG KIM, Yong-Kook MOON, Jung-Ae JI HA KIM CHUNG, You-Nam WANG, Kyu-Chang |
| description | The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD.
We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy (2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2-dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD (2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years).
We identified 1 polypeptide spot (Mr of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients (mean optical density intensity, 0.36+/-0.24; range, 0.05 to 0.92) and control spinal CSF samples (mean, 0.03+/-0.04; range, 0 to 0.08; P=0.002). This polypeptide was identified as cellular retinoic acid-binding protein (CRABP)-I. High levels of expression of CRABP-I in the CSF from 17 MMD children were confirmed by Western blotting.
The analysis of the CSF of MMD patients reveals high CRABP-I expression. The present study suggests that the elevation of CRABP-I in CSF may be a candidate for pathogenesis of MMD. |
| doi_str_mv | 10.1161/01.STR.0000100159.43123.D7 |
| format | article |
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We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy (2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2-dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD (2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years).
We identified 1 polypeptide spot (Mr of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients (mean optical density intensity, 0.36+/-0.24; range, 0.05 to 0.92) and control spinal CSF samples (mean, 0.03+/-0.04; range, 0 to 0.08; P=0.002). This polypeptide was identified as cellular retinoic acid-binding protein (CRABP)-I. High levels of expression of CRABP-I in the CSF from 17 MMD children were confirmed by Western blotting.
The analysis of the CSF of MMD patients reveals high CRABP-I expression. The present study suggests that the elevation of CRABP-I in CSF may be a candidate for pathogenesis of MMD.</description><identifier>ISSN: 0039-2499</identifier><identifier>EISSN: 1524-4628</identifier><identifier>DOI: 10.1161/01.STR.0000100159.43123.D7</identifier><identifier>PMID: 14605320</identifier><identifier>CODEN: SJCCA7</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Biological and medical sciences ; Biomarkers - analysis ; Biomarkers - cerebrospinal fluid ; Blotting, Western ; Cerebrospinal Fluid - chemistry ; Cerebrospinal Fluid Proteins - analysis ; Child ; Child, Preschool ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Male ; Medical sciences ; Moyamoya Disease - cerebrospinal fluid ; Moyamoya Disease - etiology ; Neurology ; Receptors, Retinoic Acid - analysis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Stroke (1970), 2003-12, Vol.34 (12), p.2835-2841</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-a07f2435108ecbcb2ba8d5cbbf57b481ba9b168e003dc4ca9287f6f6e4e49cfe3</citedby><cites>FETCH-LOGICAL-c434t-a07f2435108ecbcb2ba8d5cbbf57b481ba9b168e003dc4ca9287f6f6e4e49cfe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15338878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14605320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIM, Seung-Ki</creatorcontrib><creatorcontrib>YOO, Jong-Il</creatorcontrib><creatorcontrib>CHO, Byung-Kyu</creatorcontrib><creatorcontrib>SOO JIN HONG</creatorcontrib><creatorcontrib>KIM, Yong-Kook</creatorcontrib><creatorcontrib>MOON, Jung-Ae</creatorcontrib><creatorcontrib>JI HA KIM</creatorcontrib><creatorcontrib>CHUNG, You-Nam</creatorcontrib><creatorcontrib>WANG, Kyu-Chang</creatorcontrib><title>Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease</title><title>Stroke (1970)</title><addtitle>Stroke</addtitle><description>The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD.
We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy (2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2-dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD (2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years).
We identified 1 polypeptide spot (Mr of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients (mean optical density intensity, 0.36+/-0.24; range, 0.05 to 0.92) and control spinal CSF samples (mean, 0.03+/-0.04; range, 0 to 0.08; P=0.002). This polypeptide was identified as cellular retinoic acid-binding protein (CRABP)-I. High levels of expression of CRABP-I in the CSF from 17 MMD children were confirmed by Western blotting.
The analysis of the CSF of MMD patients reveals high CRABP-I expression. The present study suggests that the elevation of CRABP-I in CSF may be a candidate for pathogenesis of MMD.</description><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - cerebrospinal fluid</subject><subject>Blotting, Western</subject><subject>Cerebrospinal Fluid - chemistry</subject><subject>Cerebrospinal Fluid Proteins - analysis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Moyamoya Disease - cerebrospinal fluid</subject><subject>Moyamoya Disease - etiology</subject><subject>Neurology</subject><subject>Receptors, Retinoic Acid - analysis</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0039-2499</issn><issn>1524-4628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpFkNtOwzAMQCMEYmPwCyhCgreWXNuUNxi3SUjcn6MkdbSgrh1NB-LvyWDSLFt-Obblg9AJJTmlBT0nNH99e8lJCppSVrnglPH8utxBYyqZyETB1C4aE8KrjImqGqGDGD8Sz7iS-2hERUEkZ2SMnm8a-DJD6FrceTx9ubx6ymY4tHiYA3bQg-27uAytabBvVqFeU8vEQztE_B2GOV50P2ZduA4RTIRDtOdNE-Fo0yfo_fbmbXqfPTzezaaXD5kTXAyZIaVngktKFDjrLLNG1dJZ62VphaLWVJYWCtIPtRPOVEyVvvAFCBCV88An6Ox_77LvPlcQB70I0UHTmBa6VdQlFbTikiTw4h906ZXYg9fLPixM_6Mp0WuhmlCdhOqtUP0nVF-Xafh4c2VlF1BvRzcGE3C6AUx0pvG9aV2IW05yrlSp-C9Sqn8t</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>KIM, Seung-Ki</creator><creator>YOO, Jong-Il</creator><creator>CHO, Byung-Kyu</creator><creator>SOO JIN HONG</creator><creator>KIM, Yong-Kook</creator><creator>MOON, Jung-Ae</creator><creator>JI HA KIM</creator><creator>CHUNG, You-Nam</creator><creator>WANG, Kyu-Chang</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease</title><author>KIM, Seung-Ki ; YOO, Jong-Il ; CHO, Byung-Kyu ; SOO JIN HONG ; KIM, Yong-Kook ; MOON, Jung-Ae ; JI HA KIM ; CHUNG, You-Nam ; WANG, Kyu-Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-a07f2435108ecbcb2ba8d5cbbf57b481ba9b168e003dc4ca9287f6f6e4e49cfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biomarkers - cerebrospinal fluid</topic><topic>Blotting, Western</topic><topic>Cerebrospinal Fluid - chemistry</topic><topic>Cerebrospinal Fluid Proteins - analysis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Moyamoya Disease - cerebrospinal fluid</topic><topic>Moyamoya Disease - etiology</topic><topic>Neurology</topic><topic>Receptors, Retinoic Acid - analysis</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIM, Seung-Ki</creatorcontrib><creatorcontrib>YOO, Jong-Il</creatorcontrib><creatorcontrib>CHO, Byung-Kyu</creatorcontrib><creatorcontrib>SOO JIN HONG</creatorcontrib><creatorcontrib>KIM, Yong-Kook</creatorcontrib><creatorcontrib>MOON, Jung-Ae</creatorcontrib><creatorcontrib>JI HA KIM</creatorcontrib><creatorcontrib>CHUNG, You-Nam</creatorcontrib><creatorcontrib>WANG, Kyu-Chang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stroke (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIM, Seung-Ki</au><au>YOO, Jong-Il</au><au>CHO, Byung-Kyu</au><au>SOO JIN HONG</au><au>KIM, Yong-Kook</au><au>MOON, Jung-Ae</au><au>JI HA KIM</au><au>CHUNG, You-Nam</au><au>WANG, Kyu-Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease</atitle><jtitle>Stroke (1970)</jtitle><addtitle>Stroke</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>34</volume><issue>12</issue><spage>2835</spage><epage>2841</epage><pages>2835-2841</pages><issn>0039-2499</issn><eissn>1524-4628</eissn><coden>SJCCA7</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD.
We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy (2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2-dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD (2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years).
We identified 1 polypeptide spot (Mr of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients (mean optical density intensity, 0.36+/-0.24; range, 0.05 to 0.92) and control spinal CSF samples (mean, 0.03+/-0.04; range, 0 to 0.08; P=0.002). This polypeptide was identified as cellular retinoic acid-binding protein (CRABP)-I. High levels of expression of CRABP-I in the CSF from 17 MMD children were confirmed by Western blotting.
The analysis of the CSF of MMD patients reveals high CRABP-I expression. The present study suggests that the elevation of CRABP-I in CSF may be a candidate for pathogenesis of MMD.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>14605320</pmid><doi>10.1161/01.STR.0000100159.43123.D7</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Stroke (1970), 2003-12, Vol.34 (12), p.2835-2841 |
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| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Biological and medical sciences Biomarkers - analysis Biomarkers - cerebrospinal fluid Blotting, Western Cerebrospinal Fluid - chemistry Cerebrospinal Fluid Proteins - analysis Child Child, Preschool Electrophoresis, Gel, Two-Dimensional Female Humans Male Medical sciences Moyamoya Disease - cerebrospinal fluid Moyamoya Disease - etiology Neurology Receptors, Retinoic Acid - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Vascular diseases and vascular malformations of the nervous system |
| title | Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease |
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