Elevation of CRABP-I in the cerebrospinal fluid of patients with moyamoya disease

The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD. We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5...

Full description

Saved in:
Bibliographic Details
Published in:Stroke (1970) 2003-12, Vol.34 (12), p.2835-2841
Main Authors: KIM, Seung-Ki, YOO, Jong-Il, CHO, Byung-Kyu, SOO JIN HONG, KIM, Yong-Kook, MOON, Jung-Ae, JI HA KIM, CHUNG, You-Nam, WANG, Kyu-Chang
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers - analysis
Biomarkers - cerebrospinal fluid
Blotting, Western
Cerebrospinal Fluid - chemistry
Cerebrospinal Fluid Proteins - analysis
Child
Child, Preschool
Electrophoresis, Gel, Two-Dimensional
Female
Humans
Male
Medical sciences
Moyamoya Disease - cerebrospinal fluid
Moyamoya Disease - etiology
Neurology
Receptors, Retinoic Acid - analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Vascular diseases and vascular malformations of the nervous system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD. We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy (2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2-dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD (2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years). We identified 1 polypeptide spot (Mr of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients (mean optical density intensity, 0.36+/-0.24; range, 0.05 to 0.92) and control spinal CSF samples (mean, 0.03+/-0.04; range, 0 to 0.08; P=0.002). This polypeptide was identified as cellular retinoic acid-binding protein (CRABP)-I. High levels of expression of CRABP-I in the CSF from 17 MMD children were confirmed by Western blotting. The analysis of the CSF of MMD patients reveals high CRABP-I expression. The present study suggests that the elevation of CRABP-I in CSF may be a candidate for pathogenesis of MMD.
ISSN:0039-2499
1524-4628
DOI:10.1161/01.STR.0000100159.43123.D7