Distribution of killer cell immunoglobulin-like receptors in the Greek population

The interaction between killer cell immunoglobulin-like receptors (KIR) expressed on natural killer (NK) cells, and human leukocyt antigen (HLA) molecules expressed on target cells is known to regulate the cytolytic activity. A wide range of KIR genotypes is observed in the population, as the number...

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Bibliographic Details
Published in:Human immunology 2003-12, Vol.64 (12), p.1167-1176
Main Authors: Niokou, Dimitra, Spyropoulou-Vlachou, Maria, Darlamitsou, Areti, Stavropoulos-Giokas, Catherine
Format: Article
Language:English
Subjects:
DNA Primers - genetics
Genetic Markers - genetics
Genetic Markers - immunology
Genotype
Greece
Greek population
HLA Antigens - genetics
HLA Antigens - immunology
Humans
Killer Cells, Natural - immunology
KIR
KIR frequencies
KIR genotypes
Linkage Disequilibrium
PCR-SSP
Receptors, Immunologic - genetics
Receptors, Immunologic - immunology
Receptors, KIR
Receptors, KIR2DL1
Receptors, KIR2DL2
Receptors, KIR2DL3
Receptors, KIR2DL4
Receptors, KIR3DL1
Receptors, KIR3DL2
Receptors, KIR3DS1
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Summary:The interaction between killer cell immunoglobulin-like receptors (KIR) expressed on natural killer (NK) cells, and human leukocyt antigen (HLA) molecules expressed on target cells is known to regulate the cytolytic activity. A wide range of KIR genotypes is observed in the population, as the number of KIR loci can vary. In the present study we have determined the frequencies and combinations of 13 KIR genes and two CD94:NKG2 receptor genes and their distribution in the two haplotype groups in a panel of 233 unrelated healthy Greek Caucasians. We have typed genomic DNA for the presence of the putative KIR loci KIR2DL1, KIR2DL2, KIR2DL3, KIR2DL4, KIR3DL1, KIR3DL2, KIR3DL3, KIR2DS1, KIR2DS2, KIR2DS3, KIR2DS4, KIR2DS5, and KIR3DS1 using modified polymerase chain reaction sequence-specific primers. The frequency of KIR loci combined with the linkage disequilibrium values suggest that the Greek population shares several general features with other Caucasoid populations studied before, but still distinguishes itself by the increased or decreased frequency of several alleles. The majority of the 45 different KIR genotypes seen in Greeks have been observed in Caucasoid populations studied before. Nevertheless, two of these genotypes, although met in other populations, have not been found in Caucasians before. One individual possesses a novel profile with no KIR inhibitory gene. The A haplotypes remain the most prevalent, with AA1 being the most common genotype, and the number of inhibitory KIRs being more variable than the number of noninhibitory KIRs in most haplotypes.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2003.08.100