Adjuvant Activity of Free Bordetella Pertussis Filamentous Haemagglutinin Delivered by Mucosal Routes

The development of safe and potent mucosal adjuvants remains a major objective in vaccinology. The potential usefulness of filamentous haemagglutinin (FHA) of Bordetella pertussis as an adjuvant was assessed in a mouse model. The glutathione‐S‐transferase of Schistosoma mansoni (Sm28GST) was used fo...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2003-11, Vol.58 (5), p.503-510
Main Authors: Poulain‐Godefroy, O., Menozzi, F. D., Alonso, S., Vendeville, C., Capron, A., Locht, C., Riveau, G.
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - administration & dosage
Adhesins, Bacterial - chemistry
Adhesins, Bacterial - pharmacology
Adjuvants, Immunologic - administration & dosage
Administration, Intranasal
Animals
B7-1 Antigen - genetics
Bordetella pertussis
Female
Genes, MHC Class II
Glutathione Transferase - immunology
Hemagglutinins - administration & dosage
Hemagglutinins - chemistry
Hemagglutinins - pharmacology
Hemocyanins - immunology
Mice
Schistosoma mansoni - immunology
Transforming Growth Factor beta - genetics
Virulence Factors, Bordetella - administration & dosage
Virulence Factors, Bordetella - chemistry
Virulence Factors, Bordetella - pharmacology
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Summary:The development of safe and potent mucosal adjuvants remains a major objective in vaccinology. The potential usefulness of filamentous haemagglutinin (FHA) of Bordetella pertussis as an adjuvant was assessed in a mouse model. The glutathione‐S‐transferase of Schistosoma mansoni (Sm28GST) was used for intranasal administration, while the gut‐resistant keyhole limpet haemocyanin (KLH) was administrated by the oral route. For both antigens, coadministration with FHA increased antigen‐specific immunoglobulin titres. This adjuvant effect did not require chemical cross‐linking or direct interaction between FHA and the antigen tested. FHA also behaved as an adjuvant by the subcutaneous route, indicating that its adjuvanticity is not restricted to binding to mucosal surfaces. The FHA‐induced adjuvanticity was also observed in mice with high anti‐FHA antibody titres as a result of antipertussis vaccination, indicating that pre‐existing anti‐FHA antibodies do not impair FHA adjuvanticity. No mRNA coding for proinflammatory cytokines was induced in the lungs after intranasal FHA administration. However, an increase in the levels of mRNAs coding for B7‐1, transforming growth factor (TGF)‐β and major histocompatibility complex (MHC)‐II was detected in the lungs after FHA administration. Although the molecular mechanisms of the FHA‐induced adjuvanticity remain to be elucidated, the data presented here indicate that this adhesin, already assessed for human use as a pertussis vaccine constituent, represents a promising adjuvant to improve the humoral immune response when given by mucosal routes.
ISSN:0300-9475
1365-3083
DOI:10.1046/j.1365-3083.2003.01336.x