ABI5 Interacts with Abscisic Acid Signaling Effectors in Rice Protoplasts

Abscisic acid (ABA) regulates seed maturation, germination, and adaptation of vegetative tissues to environmental stresses. The mechanisms of ABA action and the specificity conferred by signaling components in overlapping pathways are not completely understood. The ABI5 gene (ABAinsensitive 5) of Ar...

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Bibliographic Details
Published in:The Journal of biological chemistry 2002-01, Vol.277 (3), p.1689-1694
Main Authors: Gampala, Srinivas S.L., Finkelstein, Ruth R., Sun, Samuel S.M., Rock, Christopher D.
Format: Article
Language:English
Subjects:
1-butanol
ABI5 gene
Abscisic Acid - metabolism
Arabidopsis
Arabidopsis Proteins
Basic-Leucine Zipper Transcription Factors
Cyanophyta
Freshwater
Oryza - metabolism
Oryza - ultrastructure
Oryza sativa
Plant Proteins - metabolism
Protein Binding
Protoplasts - metabolism
Signal Transduction
Transcription Factors - metabolism
VP1 protein
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Summary:Abscisic acid (ABA) regulates seed maturation, germination, and adaptation of vegetative tissues to environmental stresses. The mechanisms of ABA action and the specificity conferred by signaling components in overlapping pathways are not completely understood. The ABI5 gene (ABAinsensitive 5) of Arabidopsisencodes a basic leucine zipper factor required for ABA response in the seed and vegetative tissues. Using transient gene expression in rice protoplasts, we provide evidence for the functional interactions of ABI5 with ABA signaling effectors VP1 (viviparous 1) and ABI1 (ABA insensitive 1). Co-transformation experiments with ABI5 cDNA constructs resulted in specific transactivation of the ABA-inducible wheat Em, Arabidopsis AtEm6, bean β-Phaseolin, and barleyHVA1 and HVA22 promoters. Furthermore, ABI5 interacted synergistically with ABA and co-expressed VP1, indicating that ABI5 is involved in ABA-regulated transcription mediated by VP1. ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, a competitive inhibitor of phospholipase D involved in ABA signaling. Lanthanum, a trivalent ion that acts as an agonist of ABA signaling, potentiated ABI5 transactivation. These results demonstrate that ABI5 is a key target of a conserved ABA signaling pathway in plants.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M109980200