Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern

We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2001-05, Vol.3 (3), p.224-230
Main Authors: Maccatrozzo, L, Bargelloni, L, Radaelli, G, Mascarello, F, Patarnello, T
Format: Article
Language:English
Subjects:
Amino acids
Animal tissues
Biological evolution
Developmental stages
Fish
Gene expression
Gene sequencing
Genes
Introns
Mammals
Marine
MSTN gene
Myostatin
Nucleic acids
Nucleotide sequence
PCR
Phylogeny
Polymerase chain reaction
Proteins
Ribonucleic acid
RNA
RNA-directed DNA polymerase
Sequencing
Skeletal muscle
Sparus aurata
Tissue
Tissues
Transcription
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Summary:We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis of MSTN and other related genes confirmed the evolutionary relationships of the isolated sequence. The complete sequences of two introns were also determined. Intron-exon boundaries were conserved when compared with those of mammalian MSTN genes, whereas intron size was smaller. Reverse transcriptase polymerase chain reaction on total RNA extracted from different tissues and developmental stages revealed MSTN expression in the skeletal muscle, but also in other tissues. The observed expression profile differed from that in mammals, suggesting possible additional functions of myostatin in the teleost fish.
ISSN:1436-2228
1436-2236
DOI:10.1007/s101260000064