Biochemical Evidence for the Activation of Distinct Subsets of Mitogen-Activated Protein Kinases by Voltage and Defense-Related Stimuli

Activation of mitogen-activated protein (MAP) kinases is a common reaction of plant cells in defense-related signal transduction pathways. To gain insight into the mechanisms that determine specificity in response to a particular stimulus, a biochemical approach has been employed. Photoautotrophic s...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2002-01, Vol.128 (1), p.271-281
Main Authors: Vinzenz L. Link, Markus G. Hofmann, Alok K. Sinha, Ehness, Rainer, Strnad, Miroslav, Roitsch, Thomas
Format: Article
Language:English
Subjects:
Alfalfa
Antibodies
Antibodies - immunology
Antibodies - pharmacology
Biological and medical sciences
Calcium
Calcium - metabolism
Calcium Channel Blockers - pharmacology
Cell biochemistry
Cell Biology and Signal Transduction
Cell culture techniques
Cell physiology
Cells, Cultured
Chitin - analogs & derivatives
Chitin - pharmacology
Chitosan
Cultured cells
Electric potential
Electric Stimulation
Enzyme Activation - drug effects
Enzyme Activation - radiation effects
Fundamental and applied biological sciences. Psychology
Fusarium - growth & development
Generalities. Disease free stocks
Hydrogen Peroxide - metabolism
Immunity, Innate
Immunoprecipitation
Lycopersicon esculentum - cytology
Lycopersicon esculentum - enzymology
Lycopersicon esculentum - microbiology
Mitogen-Activated Protein Kinases - genetics
Mitogen-Activated Protein Kinases - immunology
Mitogen-Activated Protein Kinases - metabolism
Molecular and cellular biology
Nifedipine - pharmacology
Pathogens
Pectins - pharmacology
Phytopathology. Animal pests. Plant and forest protection
Plant cells
Plant Diseases - microbiology
Plant physiology and development
Plants
Precipitin Tests
RNA, Messenger - genetics
RNA, Messenger - metabolism
Salicylic Acid - pharmacology
Signal transduction
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Summary:Activation of mitogen-activated protein (MAP) kinases is a common reaction of plant cells in defense-related signal transduction pathways. To gain insight into the mechanisms that determine specificity in response to a particular stimulus, a biochemical approach has been employed. Photoautotrophic suspension culture cells of tomato (Lycopersicon peruvianum) were used as experimental system to characterize MAP kinase activation by different stress-related stimuli. An elicitor preparation of the tomato-specific pathogen Fusarium oxysporum lycopersici was shown to result in the simultaneous induction of four kinase activities that could be separated by ion-exchange chromatography. The simultaneous activation of multiple MAP kinases was further substantiated by distinct pharmacological and immunological properties: a differential sensitivity toward various protein kinase inhibitors and a differential cross-reaction with isoform-specific MAP kinase antibodies. In contrast to the two fungal elicitors chitosan and the F. oxysporum lycopersici preparation, the plant-derived stimuli polygalacturonic acid and salicylic acid were shown to activate distinctly different subsets of MAP kinases. Application of a voltage pulse was introduced as a transient stress-related stimulus that does not persist in the culture. Voltage application activates a distinct set of MAP kinases, resembling those activated by salicylic acid treatment, and generates a refractory state for the salicylic acid response. The inhibitory effect of nifedipine indicates that current application may directly affect voltage-gated calcium channels, thus, providing a tool to study various calcium-dependent pathways.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.010569