Quantitative measurement of biotin-binding immunoglobulin G in human sera using F(ab')2 anti-human IgG-coated multi-well plates

Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2003-11, Vol.26 (11), p.1605-1608
Main Authors: Muratsugu, Makoto, Muramoto, Eri, Fukui, Toru
Format: Article
Language:English
Subjects:
Binding Sites, Antibody
Biotin - blood
Dose-Response Relationship, Immunologic
Humans
Immunoglobulin G - blood
Protein Binding - immunology
Receptors, Immunologic - blood
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Summary:Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin and peroxidase-biotin, or avidin-biotinylated peroxidase complex with method A, B, or C, respectively. Commercially available B-IgG was detected by all these three methods. However, method A and B could not detect B-IgG in the human sera used in this study, but we quantitatively measured the B-IgG level using method C. The result is probably due to the fact that the sensitivity of method C was higher than that of methods A or B. Properties of B-IgG detected by method C are discussed in the text.
ISSN:0918-6158
1347-5215