Differences between Subcultures of the Mesorhizobium loti Type Strain from Different Culture Collections

Because of differences in the reported 16S rRNA gene sequence of the Mesorhizobium loti type strain available from different culture collections, we collected different subcultures of this strain and compared them by 16S rDNA sequencing, SDS-PAGE of whole-cell protein extracts and RAPD-PCR. Our resu...

Full description

Saved in:
Bibliographic Details
Published in:Systematic and applied microbiology 2001-12, Vol.24 (4), p.549-553
Main Authors: Willems, Anne, Hoste, Bart, Tang, Jane, Janssens, Danielle, Gillis, Monique
Format: Article
Language:English
Subjects:
16S rDNA sequences
Bacteriology
Biological and medical sciences
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Mesorhizobium loti
Microbiology
Phylogeny
preservation
Random Amplified Polymorphic DNA Technique
Rhizobium - chemistry
Rhizobium - classification
Rhizobium - genetics
Ribotyping
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
subculture differences
Systematics
type strain
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Because of differences in the reported 16S rRNA gene sequence of the Mesorhizobium loti type strain available from different culture collections, we collected different subcultures of this strain and compared them by 16S rDNA sequencing, SDS-PAGE of whole-cell protein extracts and RAPD-PCR. Our results indicate that the 16S rDNA sequence differences can be explained by the presence of two different organisms in one of the subcultures. In addition, even for subcultures of the type strain that had identical 16S rDNA sequences, small differences could be observed in the protein profiles and in the RAPD-PCR patterns. These latter observations indicate that maintenance procedures necessary for long-term preservation by freeze-drying can cause subcultures of the same original strain to undergo changes, effectively leading to different fingerprints even though 16S rDNA sequences remain identical.
ISSN:0723-2020
1618-0984
DOI:10.1078/0723-2020-00070