Regulation of sulfoglucuronyl glycolipid synthesis in the developing rat sciatic nerve

Sulfoglucuronyl glycolipids (SGGLs) have been considered as target antigens in demyelinating peripheral neuropathies associated with IgM monoclonal gammopathy. The regulation of expression of SGGLs in the rat sciatic nerve during development was studied by assaying the levels of SGGLs and activities...

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Bibliographic Details
Published in:Neurochemical research 2001-11, Vol.26 (11), p.1231-1235
Main Authors: CHOU, Denise K. H, JUNGALWALA, Firoze B
Format: Article
Language:English
Subjects:
Aging - physiology
Animals
Biological and medical sciences
Carbohydrate Sequence
Development. Senescence. Regeneration. Transplantation
Fundamental and applied biological sciences. Psychology
Globosides - biosynthesis
Glycolipids - biosynthesis
Glycosyltransferases - metabolism
Molecular Sequence Data
Rats
Sciatic Nerve - growth & development
Sciatic Nerve - metabolism
Vertebrates: nervous system and sense organs
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Summary:Sulfoglucuronyl glycolipids (SGGLs) have been considered as target antigens in demyelinating peripheral neuropathies associated with IgM monoclonal gammopathy. The regulation of expression of SGGLs in the rat sciatic nerve during development was studied by assaying the levels of SGGLs and activities of four glycosyltransferases sequentially involved in their synthesis from lactosylceramide. The levels of SGGLs in the sciatic nerve increased with development and reached a maximum at sixty days after birth. The rate of increase in the level of SGGLs between day 5 to 20 was similar to rate of deposition of myelin in the nerve. Analysis of the activities of the glycosyltransferases showed that only lactotriosylceramide galactosyltransferase (LcOse3Cer-GalTr) increased in parallel with the levels of SGGLs during development. The other three enzymes were not co-relative with the synthesis of SGGLs. The product of LcOse3Cer-GalTr reaction, nLcOse4Cer is the key intermediate for all neolactoglycolipids, particularly NeuAc alpha2-3nLcOse4Cer or nLM1, which is the major ganglioside (60%) of myelin in rat sciatic nerve. The results suggest that in the sciatic nerve SGGLs are mostly associated with Schwann cell myelin and their synthesis is regulated by LcOse3Cer-GalTr, unlike in the cerebral cortex and cerebellum where SGGLs are associated with the neuronal membranes and their synthesis is regulated by lactosylceramide N-acetylglucosaminyltransferase (LcOse2Cer-GlcNAcTr).
ISSN:0364-3190
1573-6903
DOI:10.1023/A:1013967323241