Genomic and antigenic variation among rotavirus strains circulating in a large city of Argentina

Knowledge of the antigenic diversity of rotaviruses circulating in a region should be acquired before introducing a rotavirus vaccine. In a collection of 151 rotavirus‐positive samples from Mendoza, Argentina, strain diversity was evaluated utilizing G‐typing monoclonal antibodies (MAbs), reverse‐tr...

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Published in:Journal of medical virology 2000-08, Vol.61 (4), p.504-509
Main Authors: Espul, Carlos, Cuello, Hector, Martinez, Norma, Centorbi, Olga, O'Ryan, Miguel, Jackson, Laurie, Campos, Francisco, Matson, David O.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
antigenic
Antigenic Variation
Antigens, Viral - analysis
Antigens, Viral - immunology
Argentina - epidemiology
Biological and medical sciences
Capsid - analysis
Capsid - immunology
Electrophoresis, Polyacrylamide Gel
Epidemiology
Feces - virology
Fundamental and applied biological sciences. Psychology
Genetic Variation
genomic
Humans
Infant
Infant, Newborn
Microbiology
Reverse Transcriptase Polymerase Chain Reaction
rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus - immunology
Rotavirus Infections - epidemiology
Rotavirus Infections - virology
strains
types
variation
Virology
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Summary:Knowledge of the antigenic diversity of rotaviruses circulating in a region should be acquired before introducing a rotavirus vaccine. In a collection of 151 rotavirus‐positive samples from Mendoza, Argentina, strain diversity was evaluated utilizing G‐typing monoclonal antibodies (MAbs), reverse‐transcriptase‐polymerase chain reaction (RT‐PCR) G and P typing, and electropherotyping (PAGE). The G type of 137 (91%) specimens was determined. Typing MAb reactivity with the homologous type ranged from 25–94%. For the seven G1 MAbs utilized, 28 patterns of reactivity among 68 G1 strains occurred. For the 48 G2 strains, six patterns of reactivity occurred utilizing three G2‐specific MAbs. Of the 92 samples G‐ and P‐typed by reverse‐transcriptase‐polymerase chain reaction, 89% had single G/P combinations: eight G1[P4], one G1[P6], twelve G1[P8], 58 G2 [P4], and two G2 [P6]. Nine samples had more than one G type with a single P type, one sample had two P types associated with one G type, and one sample contained multiple G and P types. Twenty‐nine PAGE patterns occurred for all G types, but differences of antigenic reaction did not predict differences in migration of gene segments 7, 8, and 9. For three specimens showing discordant results between G type by enzyme‐linked immunosorbent assay (EIA) and RT‐PCR, we observed unexpected electropherotypes. Complementary evaluation by RT‐PCR and MAb‐based EIA with multiple typing MAbs revealed genetic and antigenic diversity of circulating rotaviruses, including extensive intratypic variation of the G1 and G2 neutralization antigens, in Mendoza during a single season of rotavirus activity. J. Med. Virol. 61:504–509, 2000. © 2000 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/1096-9071(200008)61:4<504::AID-JMV14>3.0.CO;2-S