Direct and Highly Species-Specific Detection of Pork Meat and Fat in Meat Products by PCR Amplification of Mitochondrial DNA

Highly species-specific primers for pork D-loop mtDNA have been designed. Use of these and restrictive PCR amplification conditions has improved a reliable and rapid method for detecting a PCR-amplified 531 bp band from pork. It has been proved useful for detecting both pork meat and fat in meat mix...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2000-07, Vol.48 (7), p.2829-2832
Main Authors: Montiel-Sosa, J. F, Ruiz-Pesini, E, Montoya, J, Roncalés, P, López-Pérez, M. J, Pérez-Martos, A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
DNA, Mitochondrial - chemistry
Food industries
Fundamental and applied biological sciences. Psychology
Gene Amplification
Humans
Meat and meat product industries
Meat Products
Polymerase Chain Reaction
Species Specificity
Swine
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Summary:Highly species-specific primers for pork D-loop mtDNA have been designed. Use of these and restrictive PCR amplification conditions has improved a reliable and rapid method for detecting a PCR-amplified 531 bp band from pork. It has been proved useful for detecting both pork meat and fat in meat mixtures, including those dry-cured and heated by cooking. Absence of response in PCR-amplified samples or mixtures from bovine, ovine, chicken, and human was also demonstrated. Furthermore, wild boar and pork samples can be also easily distinguished by a simple AvaII restriction analysis. Keywords: Mitochondrial DNA; PCR analysis; pork; meat; meat products; species identification
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9907438