Time-dependent enhancement of inhibitory avoidance retention and MAPK activation by post-training infusion of nerve growth factor into CA1 region of hippocampus of adult rats

Several studies have demonstrated that chronic intracerebroventricular nerve growth factor (NGF) infusion has a beneficial effect on cognitive performance of lesioned as well as old and developing animals. Here we investigate: (i) the effect of post‐training infusion of NGF into the CA1 region of hi...

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Published in:The European journal of neuroscience 2000-06, Vol.12 (6), p.2185-2189
Main Authors: Walz, Roger, Lenz, Guido, Roesler, Rafael, Vianna, Mônica M. R., Martins, Vilma, Brentani, Ricardo, Rodnight, Richard, Izquierdo, Ivan
Format: Article
Language:English
Subjects:
Age Factors
Animals
Avoidance Learning - drug effects
Avoidance Learning - physiology
Cognition - physiology
cognitive performance
ERK
hippocampal microslices
Hippocampus - drug effects
Hippocampus - enzymology
Male
MAP Kinase Signaling System - physiology
mitogen-activated protein kinase
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases - metabolism
Nerve Growth Factor - pharmacology
Organ Culture Techniques
p42/p44 MAPK
Rats
Rats, Wistar
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Summary:Several studies have demonstrated that chronic intracerebroventricular nerve growth factor (NGF) infusion has a beneficial effect on cognitive performance of lesioned as well as old and developing animals. Here we investigate: (i) the effect of post‐training infusion of NGF into the CA1 region of hippocampus on inhibitory avoidance (IA) retention in rats; (ii) the extension of the effect, in time and space, of NGF infusion into CA1 on the activity of mitogen‐activated protein kinase (MAPK, syn: ERK1/2, p42/p44 MAPK). NGF was bilaterally injected into the CA1 regions of the dorsal hippocampus (0.05, 0.5 or 5.0 ng diluted in 0.5 μL of saline per side) at 0, 120 or 360 min after IA training in rats. Retention testing was carried out 24 h after training. The injection of 5.0 and 0.5, but not 0.05, ng per side of NGF at 0 and 120 min after IA training enhanced IA retention. The highest dose used was ineffective when injected 360 min after training. The infusion of 0.5 μL of NGF (5.0 ng) induced a significant enhancement of MAPK activity in hippocampal microslices; this enhancement was restricted to a volume with 0.8 mm radius at 30 min after injection. The MAPK activation was still seen 180 min after NGF infusion, although this value showed only a tendency. In conclusion, localized infusion of NGF into the CA1 region enhanced MAPK activity, restricted in time and space, and enhanced IA retention in a time‐ and dose‐dependent manner.
ISSN:0953-816X
1460-9568
DOI:10.1046/j.1460-9568.2000.00123.x