β-Adrenergic stimulation restores rat lung ability to clear edema in ventilator-associated lung injury

Mechanical ventilation with high tidal volume (HVT) causes lung injury and decreases the lung's ability to clear edema in rats. beta-adrenergic agonists increase active Na(+) transport and lung edema clearance in normal rat lungs by stimulating apical Na(+) channels and basolateral Na,K-ATPase...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2000-07, Vol.162 (1), p.282-287
Main Authors: SALDIAS, F. J, LECUONA, E, COMELLAS, A. P, RIDGE, K. M, RUTSCHMAN, D. H, SZNAJDER, J. I
Format: Article
Language:English
Subjects:
Adrenergic beta-Agonists - pharmacokinetics
Adrenergic beta-Agonists - therapeutic use
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Emergency and intensive respiratory care
Intensive care medicine
Isoproterenol - pharmacokinetics
Isoproterenol - therapeutic use
Lung Injury
Male
Medical sciences
Permeability
Pharmacology. Drug treatments
Pulmonary Edema - drug therapy
Pulmonary Edema - etiology
Rats
Rats, Sprague-Dawley
Respiration, Artificial - adverse effects
Respiratory Mucosa - metabolism
Respiratory system
Terbutaline - pharmacokinetics
Terbutaline - therapeutic use
Tropical medicine
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Summary:Mechanical ventilation with high tidal volume (HVT) causes lung injury and decreases the lung's ability to clear edema in rats. beta-adrenergic agonists increase active Na(+) transport and lung edema clearance in normal rat lungs by stimulating apical Na(+) channels and basolateral Na,K-ATPase in alveolar epithelial cells. We studied whether beta-adrenergic agonists could restore lung edema clearance in rats ventilated with HVT (40 ml/kg, peak airway pressure of 35 cm H(2)O) for 40 min. The ability of rat lungs to clear edema decreased by approximately 50% after 40 min of HVT ventilation. Terbutaline (TERB) and isoproterenol (ISO) increased lung edema clearance in control nonventilated rats (from 0.50 +/- 0. 02 ml/h to 0.81 +/- 0.04 ml/h and 0.99 +/- 0.05 ml/h, respectively) and restored the lung's ability to clear edema in HVT ventilated rats (from 0.25 +/- 0.03 ml/h to 0.64 +/- 0.02 ml/h and 0.88 +/- 0. 09 ml/h, respectively). Disruption of cell microtubular transport system by colchicine inhibited the stimulatory effects of ISO in HVT ventilated rats, whereas beta-lumicolchicine did not affect beta-adrenergic stimulation. The Na,K-ATPase alpha(1)- and beta(1)-subunit mRNA steady state levels were not affected by incubation with ISO for 60 min in alveolar type II cells isolated from control and HVT ventilated rats. The data suggest that beta-adrenergic agonists increased alveolar fluid reabsorption in rats ventilated with HVT by promoting recruitment of ion-transporting proteins from intracellular pools to the plasma membrane of alveolar epithelial cells.
ISSN:1073-449X
1535-4970
DOI:10.1164/ajrccm.162.1.9809058