Peripheral nerve regeneration using silicone rubber chambers filled with collagen, laminin and fibronectin

A 10 mm gap of rat sciatic nerve was created between the proximal and distal nerve stumps, which were sutured into silicone rubber tubes filled with an extracellular gel containing collagen, laminin and fibronectin. Empty silicone rubber tubes were used as controls. Six weeks after implantation, all...

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Bibliographic Details
Published in:Biomaterials 2000-08, Vol.21 (15), p.1541-1547
Main Authors: Chen, Yueh-Sheng, Hsieh, Ching-Liang, Tsai, Chin-Chuan, Chen, Ter-Hsin, Cheng, Wen-Chiang, Hu, Cheng-Li, Yao, Chun-Hsu
Format: Article
Language:English
Subjects:
Absorption
Action Potentials
Animals
Biocompatible Materials
Biodegradation
Collagen
Extracellular gel
Fibronectin
Fibronectins
Gels
Laminin
Male
Muscle, Skeletal - innervation
Muscle, Skeletal - physiology
Nerve Fibers - physiology
Nerve Fibers - ultrastructure
Nerve regeneration
Nerve Regeneration - physiology
Prostheses and Implants
Rats
Rats, Sprague-Dawley
Rubber
Sciatic Nerve - physiology
Silicone rubber
Silicones
Tissue culture
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Summary:A 10 mm gap of rat sciatic nerve was created between the proximal and distal nerve stumps, which were sutured into silicone rubber tubes filled with an extracellular gel containing collagen, laminin and fibronectin. Empty silicone rubber tubes were used as controls. Six weeks after implantation, all extracellular elements were completely degraded and absorbed, and 90% of the animals from the extracellular gel group exhibited regeneration across the nerve gaps, whereas only 60% in the control group. Both qualitative and quantitative histology of the regenerated nerves revealed a more mature ultrastructural organization with 28% larger cross-sectional area and 28% higher number of myelinated axons in the extracellular gel group than the controls. These results showed that the gel mixture of collagen, laminin and fibronectin could offer a suitable growth medium for the regeneration of axons.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(00)00028-4