Loading…
Organotypic cultures of gingival cells: an epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions
This article explores whether organotypic cultures of immortalized gingival keratinocytes constitute a suitable model for assessing the epithelial cell compatibility of two groups of dental resins, each of them representing one group used in orthodontics and temporo-mandibular disorders (TMD) therap...
Saved in:
| Published in: | Biomaterials 2000-08, Vol.21 (15), p.1549-1559 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3 |
| container_end_page | 1559 |
| container_issue | 15 |
| container_start_page | 1549 |
| container_title | Biomaterials |
| container_volume | 21 |
| creator | Tomakidi, P Schuster, G Breitkreutz, D Kohl, A Ottl, P Komposch, G |
| description | This article explores whether organotypic cultures of immortalized gingival keratinocytes constitute a suitable model for assessing the epithelial cell compatibility of two groups of dental resins, each of them representing one group used in orthodontics and temporo-mandibular disorders (TMD) therapy under conditions more closely resembling the actual tissue situation. The resins were tested with the agar diffusion assay (ADA) in conventional monolayer and organotypic cultures. Compared to the control exhibiting a neutral red destaining index of 3, the index of 4 obtained after exposure of monolayers to one soft permanent resin (Durabase
TM) indicated the presence of a non-lytic but physiologically active substance. In contrast, the adaptation of the ADA to organotypic cultures revealed no apparent lesions at the epithelial surface by performing scanning electron microscopy, while histoarchitecture indicated the development of stratified surface epithelia. This was substantiated by undamaged cells in the uppermost cell layers and by the preservation of cell-to-cell contacts. Furthermore, indirect immunofluorescence for Ki-67 and the cytokeratins ck14 and ck4 revealed that cell proliferation and epithelial structure were maintained, while differentiation was enhanced, possibly increasing epithelial resistance. The results obtained from the organotypic cultures suggest that (i) cell-affecting effects of materials visible in monolayer cultures may not be seen in epithelia resembling that in vivo and that (ii) enhanced differentiation may be associated with increased stability of the epithelial cells. Thus, organotypic cultures of gingival cells constitute a tissue model allowing short-term tissue compatibility studies of dental materials and rendering a potential candidate also for long-term studies. |
| doi_str_mv | 10.1016/S0142-9612(00)00037-5 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71229670</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0142961200000375</els_id><sourcerecordid>27705268</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3</originalsourceid><addsrcrecordid>eNqFkc-OFCEQh4nRuOPqI2g4GT20At0N3V6M2fgv2WQP7p3QUD2LMtBS9CT7WL6hzMzGeBsuBOqrXyX1EfKSs3eccfn-B-OdaEbJxRvG3jLGWtX0j8iGD2po-pH1j8nmH3JBniH-ZPXNOvGUXHA2DL0SakP-3OStiancL95Su4ayZkCaZrr1cev3JlALIeAHaiKFxZc7CL5-7pKDQEuiBhEQ6bIWU_weaEi2lmGewZZjTsrlLrkUS81fgilQkxxN1oYVK4lL8LHQXS3kGox0jQ5yzc9Ai0dcoQn-F1CbovPFp4jPyZO5gvDi4b4kt18-3159a65vvn6_-nTd2E60pZEwgBDTCPUo7uQoZwXSzfM0mRZMK6B1g5ST6eTUz0xZBcM8VUJ0UgnXXpLXp9glp98rYNE7j4ddmAhpRa24EKNU7CwolGK9kMN5kHfd2I5dBfsTaHNCzDDrJfudyfeaM32Qr4_y9cGsZkwf5eu-9r16GLBOO3D_dZ1sV-DjCYC6t72HrNF6iBacz9WXdsmfGfEXjKPEmA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21449394</pqid></control><display><type>article</type><title>Organotypic cultures of gingival cells: an epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions</title><source>ScienceDirect Freedom Collection</source><creator>Tomakidi, P ; Schuster, G ; Breitkreutz, D ; Kohl, A ; Ottl, P ; Komposch, G</creator><creatorcontrib>Tomakidi, P ; Schuster, G ; Breitkreutz, D ; Kohl, A ; Ottl, P ; Komposch, G</creatorcontrib><description>This article explores whether organotypic cultures of immortalized gingival keratinocytes constitute a suitable model for assessing the epithelial cell compatibility of two groups of dental resins, each of them representing one group used in orthodontics and temporo-mandibular disorders (TMD) therapy under conditions more closely resembling the actual tissue situation. The resins were tested with the agar diffusion assay (ADA) in conventional monolayer and organotypic cultures. Compared to the control exhibiting a neutral red destaining index of 3, the index of 4 obtained after exposure of monolayers to one soft permanent resin (Durabase
TM) indicated the presence of a non-lytic but physiologically active substance. In contrast, the adaptation of the ADA to organotypic cultures revealed no apparent lesions at the epithelial surface by performing scanning electron microscopy, while histoarchitecture indicated the development of stratified surface epithelia. This was substantiated by undamaged cells in the uppermost cell layers and by the preservation of cell-to-cell contacts. Furthermore, indirect immunofluorescence for Ki-67 and the cytokeratins ck14 and ck4 revealed that cell proliferation and epithelial structure were maintained, while differentiation was enhanced, possibly increasing epithelial resistance. The results obtained from the organotypic cultures suggest that (i) cell-affecting effects of materials visible in monolayer cultures may not be seen in epithelia resembling that in vivo and that (ii) enhanced differentiation may be associated with increased stability of the epithelial cells. Thus, organotypic cultures of gingival cells constitute a tissue model allowing short-term tissue compatibility studies of dental materials and rendering a potential candidate also for long-term studies.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(00)00037-5</identifier><identifier>PMID: 10885727</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Acrylic Resins ; Agar diffusion assay (ADA) ; Animals ; Bioassay ; Biocompatibility ; Biocompatible Materials ; Cell culture ; Cell Transformation, Viral ; Cell- and tissue-compatibility ; Epithelial differentiation ; Fluorescence ; Gingiva - cytology ; Humans ; Immunology ; Keratinocytes - cytology ; L Cells (Cell Line) ; Mice ; Occlusal Splints ; Oncogene Proteins, Viral - genetics ; Organ Culture Techniques - methods ; Organotypic culture ; Orthodontic Appliances ; Papillomaviridae - genetics ; Papillomavirus E7 Proteins ; Proliferation ; Repressor Proteins ; Scanning electron microscopy</subject><ispartof>Biomaterials, 2000-08, Vol.21 (15), p.1549-1559</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3</citedby><cites>FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10885727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tomakidi, P</creatorcontrib><creatorcontrib>Schuster, G</creatorcontrib><creatorcontrib>Breitkreutz, D</creatorcontrib><creatorcontrib>Kohl, A</creatorcontrib><creatorcontrib>Ottl, P</creatorcontrib><creatorcontrib>Komposch, G</creatorcontrib><title>Organotypic cultures of gingival cells: an epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>This article explores whether organotypic cultures of immortalized gingival keratinocytes constitute a suitable model for assessing the epithelial cell compatibility of two groups of dental resins, each of them representing one group used in orthodontics and temporo-mandibular disorders (TMD) therapy under conditions more closely resembling the actual tissue situation. The resins were tested with the agar diffusion assay (ADA) in conventional monolayer and organotypic cultures. Compared to the control exhibiting a neutral red destaining index of 3, the index of 4 obtained after exposure of monolayers to one soft permanent resin (Durabase
TM) indicated the presence of a non-lytic but physiologically active substance. In contrast, the adaptation of the ADA to organotypic cultures revealed no apparent lesions at the epithelial surface by performing scanning electron microscopy, while histoarchitecture indicated the development of stratified surface epithelia. This was substantiated by undamaged cells in the uppermost cell layers and by the preservation of cell-to-cell contacts. Furthermore, indirect immunofluorescence for Ki-67 and the cytokeratins ck14 and ck4 revealed that cell proliferation and epithelial structure were maintained, while differentiation was enhanced, possibly increasing epithelial resistance. The results obtained from the organotypic cultures suggest that (i) cell-affecting effects of materials visible in monolayer cultures may not be seen in epithelia resembling that in vivo and that (ii) enhanced differentiation may be associated with increased stability of the epithelial cells. Thus, organotypic cultures of gingival cells constitute a tissue model allowing short-term tissue compatibility studies of dental materials and rendering a potential candidate also for long-term studies.</description><subject>Acrylic Resins</subject><subject>Agar diffusion assay (ADA)</subject><subject>Animals</subject><subject>Bioassay</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials</subject><subject>Cell culture</subject><subject>Cell Transformation, Viral</subject><subject>Cell- and tissue-compatibility</subject><subject>Epithelial differentiation</subject><subject>Fluorescence</subject><subject>Gingiva - cytology</subject><subject>Humans</subject><subject>Immunology</subject><subject>Keratinocytes - cytology</subject><subject>L Cells (Cell Line)</subject><subject>Mice</subject><subject>Occlusal Splints</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Organ Culture Techniques - methods</subject><subject>Organotypic culture</subject><subject>Orthodontic Appliances</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomavirus E7 Proteins</subject><subject>Proliferation</subject><subject>Repressor Proteins</subject><subject>Scanning electron microscopy</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkc-OFCEQh4nRuOPqI2g4GT20At0N3V6M2fgv2WQP7p3QUD2LMtBS9CT7WL6hzMzGeBsuBOqrXyX1EfKSs3eccfn-B-OdaEbJxRvG3jLGWtX0j8iGD2po-pH1j8nmH3JBniH-ZPXNOvGUXHA2DL0SakP-3OStiancL95Su4ayZkCaZrr1cev3JlALIeAHaiKFxZc7CL5-7pKDQEuiBhEQ6bIWU_weaEi2lmGewZZjTsrlLrkUS81fgilQkxxN1oYVK4lL8LHQXS3kGox0jQ5yzc9Ai0dcoQn-F1CbovPFp4jPyZO5gvDi4b4kt18-3159a65vvn6_-nTd2E60pZEwgBDTCPUo7uQoZwXSzfM0mRZMK6B1g5ST6eTUz0xZBcM8VUJ0UgnXXpLXp9glp98rYNE7j4ddmAhpRa24EKNU7CwolGK9kMN5kHfd2I5dBfsTaHNCzDDrJfudyfeaM32Qr4_y9cGsZkwf5eu-9r16GLBOO3D_dZ1sV-DjCYC6t72HrNF6iBacz9WXdsmfGfEXjKPEmA</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Tomakidi, P</creator><creator>Schuster, G</creator><creator>Breitkreutz, D</creator><creator>Kohl, A</creator><creator>Ottl, P</creator><creator>Komposch, G</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Organotypic cultures of gingival cells: an epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions</title><author>Tomakidi, P ; Schuster, G ; Breitkreutz, D ; Kohl, A ; Ottl, P ; Komposch, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acrylic Resins</topic><topic>Agar diffusion assay (ADA)</topic><topic>Animals</topic><topic>Bioassay</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials</topic><topic>Cell culture</topic><topic>Cell Transformation, Viral</topic><topic>Cell- and tissue-compatibility</topic><topic>Epithelial differentiation</topic><topic>Fluorescence</topic><topic>Gingiva - cytology</topic><topic>Humans</topic><topic>Immunology</topic><topic>Keratinocytes - cytology</topic><topic>L Cells (Cell Line)</topic><topic>Mice</topic><topic>Occlusal Splints</topic><topic>Oncogene Proteins, Viral - genetics</topic><topic>Organ Culture Techniques - methods</topic><topic>Organotypic culture</topic><topic>Orthodontic Appliances</topic><topic>Papillomaviridae - genetics</topic><topic>Papillomavirus E7 Proteins</topic><topic>Proliferation</topic><topic>Repressor Proteins</topic><topic>Scanning electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomakidi, P</creatorcontrib><creatorcontrib>Schuster, G</creatorcontrib><creatorcontrib>Breitkreutz, D</creatorcontrib><creatorcontrib>Kohl, A</creatorcontrib><creatorcontrib>Ottl, P</creatorcontrib><creatorcontrib>Komposch, G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomakidi, P</au><au>Schuster, G</au><au>Breitkreutz, D</au><au>Kohl, A</au><au>Ottl, P</au><au>Komposch, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organotypic cultures of gingival cells: an epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>21</volume><issue>15</issue><spage>1549</spage><epage>1559</epage><pages>1549-1559</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>This article explores whether organotypic cultures of immortalized gingival keratinocytes constitute a suitable model for assessing the epithelial cell compatibility of two groups of dental resins, each of them representing one group used in orthodontics and temporo-mandibular disorders (TMD) therapy under conditions more closely resembling the actual tissue situation. The resins were tested with the agar diffusion assay (ADA) in conventional monolayer and organotypic cultures. Compared to the control exhibiting a neutral red destaining index of 3, the index of 4 obtained after exposure of monolayers to one soft permanent resin (Durabase
TM) indicated the presence of a non-lytic but physiologically active substance. In contrast, the adaptation of the ADA to organotypic cultures revealed no apparent lesions at the epithelial surface by performing scanning electron microscopy, while histoarchitecture indicated the development of stratified surface epithelia. This was substantiated by undamaged cells in the uppermost cell layers and by the preservation of cell-to-cell contacts. Furthermore, indirect immunofluorescence for Ki-67 and the cytokeratins ck14 and ck4 revealed that cell proliferation and epithelial structure were maintained, while differentiation was enhanced, possibly increasing epithelial resistance. The results obtained from the organotypic cultures suggest that (i) cell-affecting effects of materials visible in monolayer cultures may not be seen in epithelia resembling that in vivo and that (ii) enhanced differentiation may be associated with increased stability of the epithelial cells. Thus, organotypic cultures of gingival cells constitute a tissue model allowing short-term tissue compatibility studies of dental materials and rendering a potential candidate also for long-term studies.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>10885727</pmid><doi>10.1016/S0142-9612(00)00037-5</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2000-08, Vol.21 (15), p.1549-1559 |
| issn | 0142-9612 1878-5905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71229670 |
| source | ScienceDirect Freedom Collection |
| subjects | Acrylic Resins Agar diffusion assay (ADA) Animals Bioassay Biocompatibility Biocompatible Materials Cell culture Cell Transformation, Viral Cell- and tissue-compatibility Epithelial differentiation Fluorescence Gingiva - cytology Humans Immunology Keratinocytes - cytology L Cells (Cell Line) Mice Occlusal Splints Oncogene Proteins, Viral - genetics Organ Culture Techniques - methods Organotypic culture Orthodontic Appliances Papillomaviridae - genetics Papillomavirus E7 Proteins Proliferation Repressor Proteins Scanning electron microscopy |
| title | Organotypic cultures of gingival cells: an epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-23T05%3A22%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Organotypic%20cultures%20of%20gingival%20cells:%20an%20epithelial%20model%20to%20assess%20putative%20local%20effects%20of%20orthodontic%20plate%20and%20occlusal%20splint%20materials%20under%20more%20tissue-like%20conditions&rft.jtitle=Biomaterials&rft.au=Tomakidi,%20P&rft.date=2000-08-01&rft.volume=21&rft.issue=15&rft.spage=1549&rft.epage=1559&rft.pages=1549-1559&rft.issn=0142-9612&rft.eissn=1878-5905&rft_id=info:doi/10.1016/S0142-9612(00)00037-5&rft_dat=%3Cproquest_cross%3E27705268%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c423t-6e8e22b9eeee71d696f7e6dffbba3ea32e3d866ba46b5f07c7e8fbe6d24672d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=21449394&rft_id=info:pmid/10885727&rfr_iscdi=true |