Comparative genomic hybridization of microdissected samples from different stages in the development of a seminoma and a non-seminoma

Human testicular germ cell tumours (TGCTs) of adolescents and adults, both seminomas and non‐seminomas, originate from intratubular germ cell neoplasia (IGCN). Comparative genomic hybridization (CGH) was applied to microdissected samples from different stages of the development of a seminoma and a m...

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Bibliographic Details
Published in:The Journal of pathology 2000-06, Vol.191 (2), p.187-192
Main Authors: Looijenga, Leendert H. J., Rosenberg, Carla, van Gurp, Ruud J. H. L. M., Geelen, Eric, van Echten-Arends, Jannie, de Jong, Bauke, Mostert, Marijke, Wolter Oosterhuis, J.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Case-Control Studies
Chromosome Aberrations
Chromosomes, Human - genetics
comparative genomic hybridization
DNA - genetics
Gene Amplification
Humans
Karyotyping
Male
microdissection
Middle Aged
non-seminoma
Nucleic Acid Hybridization
seminoma
Seminoma - genetics
testicular germ cell tumours
Testicular Neoplasms - genetics
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Summary:Human testicular germ cell tumours (TGCTs) of adolescents and adults, both seminomas and non‐seminomas, originate from intratubular germ cell neoplasia (IGCN). Comparative genomic hybridization (CGH) was applied to microdissected samples from different stages of the development of a seminoma and a mixed non‐seminoma, including IGCN of both. The different stages of the seminoma development, namely IGCN, intratubular and invasive seminoma, showed a very similar pattern of chromosomal imbalances, including gains of parts of 7, 8,12,14, and X, and losses of parts of 3, 4, 5, 10, 11, 12q, 16, 18, 22, and Y. A more heterogeneous pattern was found for the non‐seminoma. Some aberrations were present only in IGCN, or in IGCN and in all invasive components (gains of parts of 1q, 17, 19p, 20q, and 22, and losses of parts of 4, 5, 9p, 13, and 18q), while others were present in a less consistent pattern. These are the first reported CGH data from different stages in the development of TGCTs. Although only two cases were studied, the results suggest that particular numerical changes of (parts of) chromosomes are involved in the early development and progression of this cancer. Copyright © 2000 John Wiley & Sons, Ltd.
ISSN:0022-3417
1096-9896
DOI:10.1002/(SICI)1096-9896(200006)191:2<187::AID-PATH584>3.0.CO;2-T