Temperature Measurement in Microfluidic Systems Using a Temperature-Dependent Fluorescent Dye

A technique is described for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. The technique is easy to implement with a standard fluorescence microscope and CCD camera. In addition, the method can be used to measure fluid temperatures with mi...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-09, Vol.73 (17), p.4117-4123
Main Authors: Ross, David, Gaitan, Michael, Locascio, Laurie E
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemical and thermal methods
Chemistry
Exact sciences and technology
Fluorescence
Scientific imaging
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A technique is described for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. The technique is easy to implement with a standard fluorescence microscope and CCD camera. In addition, the method can be used to measure fluid temperatures with micrometer spatial resolution and millisecond time resolution. The efficacy of the method is demonstrated by measuring temperature distributions resulting from Joule heating in a variety of microfluidic circuits that are electrokinetically pumped. With the equipment used for these measurements, fluid temperatures ranging from room temperature to 90 °C were measured with a precision ranging from 0.03 to 3.5 °Cdependent on the amount of signal averaging done. The spatial and temporal resolutions achieved were 1 μm and 33 ms, respectively.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac010370l