alpha-Amylase production by free and immobilized Bacillus subtilis

The effect of glucose on the alpha-amylase production by Bacillus subtilis ATCC-21556 was studied. Initial glucose concentrations up to 20 g/L were found to be directly proportional to the specific alpha-amylase production in an immobilized-cell batch system, whereas a free-cell batch system present...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2000, Vol.84 (1-9), p.479-485
Main Authors: Duran-Paramo, E, Garcia-Kirchner, O, Hervagault, J.F, Thomas, D, Barbotin, J.N
Format: Article
Language:English
Subjects:
alpha-amylase
alpha-Amylases - biosynthesis
Bacillus subtilis
Bacillus subtilis - drug effects
Bacillus subtilis - enzymology
Bacillus subtilis - growth & development
batch systems
Biological and medical sciences
Biomass
Biotechnology
Cells, Immobilized - drug effects
Cells, Immobilized - enzymology
culture media
Enzyme engineering
Enzyme Induction
Fermentation
Fundamental and applied biological sciences. Psychology
glucose
Glucose - pharmacology
immobilized cells
Kinetics
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:The effect of glucose on the alpha-amylase production by Bacillus subtilis ATCC-21556 was studied. Initial glucose concentrations up to 20 g/L were found to be directly proportional to the specific alpha-amylase production in an immobilized-cell batch system, whereas a free-cell batch system presented an inversely proportional relationship with the initial glucose concentration. This might be owing to the alpha-amylase repression by the glucose present in the culture medium. Three hundred eighty-five percent of the specific alpha-amylase production with the free-cell system was produced by the immobilized-cell batch culture.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:84-86:1-9:479