Losses of Chromosomal Arms 1p and 19q in the Diagnosis of Oligodendroglioma. A Study of Paraffin-Embedded Sections

Comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), polymerase chain reaction–based microsatellite analysis, and p53 sequencing were performed in paraffin-embedded material from 18 oligodendrogliomas and histologically similar astrocytomas. The study was undertaken be...

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Bibliographic Details
Published in:Modern pathology 2001-09, Vol.14 (9), p.842-853
Main Authors: Burger, Peter C., Minn, A. Yuriko, Smith, Justin S., Borell, Thomas J., Jedlicka, Anne E., Huntley, Brenda K., Goldthwaite, Patricia T., Jenkins, Robert B., Feuerstein, Burt G.
Format: Article
Language:English
Subjects:
Adult
Aged
Astrocytoma
Brain Neoplasms - diagnosis
Brain Neoplasms - genetics
Brain Neoplasms - metabolism
Chromosome Deletion
Chromosomes, Human, Pair 1 - genetics
Chromosomes, Human, Pair 19 - genetics
Comparative genomic hybridization
Diagnosis, Differential
DNA Mutational Analysis
DNA, Neoplasm - chemistry
DNA, Neoplasm - genetics
Female
FISH
Genetics
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Male
Microtomy
Middle Aged
Mutation
Nucleic Acid Hybridization
Oligodendroglioma
Oligodendroglioma - diagnosis
Oligodendroglioma - genetics
Oligodendroglioma - metabolism
Paraffin Embedding
Tumor Suppressor Protein p53 - analysis
Tumor Suppressor Protein p53 - genetics
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Summary:Comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), polymerase chain reaction–based microsatellite analysis, and p53 sequencing were performed in paraffin-embedded material from 18 oligodendrogliomas and histologically similar astrocytomas. The study was undertaken because of evidence that concurrent loss of both the 1p and 19q chromosome arms is a specific marker for oligodendrogliomas. Of the six lesions with a review diagnosis of oligodendroglioma, all had the predicted loss of 1p and 19q seen by CGH, FISH, and polymerase chain reaction. Other lesions, including some considered oligodendroglioma or mixed glioma by the submitting institution, did not. There were no p53 mutations in any of the six oligodendrogliomas, whereas 5 of the 10 remaining, successfully studied cases did have p53 mutations. The results suggest that CGH and FISH performed on current or archival tissue can aid in classification of infiltrating gliomas such as oligodendrogliomas and astrocytomas. The results of the p53 studies are consistent with findings of previous investigations that such mutations are less common in oligodendrogliomas than they are in astrocytomas.
ISSN:0893-3952
1530-0285
DOI:10.1038/modpathol.3880400