Embryos with high implantation potential after intracytoplasmic sperm injection can be recognized by a simple, non-invasive examination of pronuclear morphology

Embryos are conventionally selected for transfer based on the evaluation of the cleavage speed and extent of blastomere fragmentation. Here we examined whether the predictive value of these criteria, as indicators of the chance of embryo implantation, can be further potentiated by adding previously...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2000-06, Vol.15 (6), p.1396-1399
Main Authors: Tesarik, J., Junca, A.M., Hazout, A., Aubriot, F.X., Nathan, C., Cohen-Bacrie, P., Dumont-Hassan, M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Nucleus - ultrastructure
Cleavage Stage, Ovum
Embryo Implantation
Embryo Transfer
Embryo, Mammalian - physiology
Embryo, Mammalian - ultrastructure
Female
Fundamental and applied biological sciences. Psychology
Humans
implantation rate
Mother. Fetoplacental unit. Mammary gland. Milk
multiple pregnancy rate
Pregnancy
Pregnancy Rate
Pregnancy, Multiple
Pregnancy. Parturition. Lactation
pronuclear morphology
single-embryo transfer
Sperm Injections, Intracytoplasmic
Vertebrates: reproduction
Zygote - ultrastructure
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Summary:Embryos are conventionally selected for transfer based on the evaluation of the cleavage speed and extent of blastomere fragmentation. Here we examined whether the predictive value of these criteria, as indicators of the chance of embryo implantation, can be further potentiated by adding previously described criteria reflecting the regularity of pronuclear development. In a group of embryos selected for transfer in 380 fresh embryo transfer cycles according to the conventional criteria, the transfer of only those embryos that developed from zygotes judged normal at the pronuclear stage (pattern 0) gave significantly higher pregnancy (44.8%) and implantation (30.2%) rates compared with the pregnancy (22.1%; P < 0.05) and implantation rates (11.2%; P < 0.001) for the transfers of only those embryos that developed from zygotes judged abnormal (non-pattern 0). The transfer of only one pattern 0 embryo was sufficient for the optimal chance of pregnancy (no differences in pregnancy rates after transfer of one, two or three pattern 0 embryos), whereas the transfer of two pattern 0 embryos mostly resulted in a twin pregnancy. The inclusion of the criteria based on pronuclear morphology can thus lead to the application of a single embryo transfer policy and optimize the selection of embryos for transfer and cryopreservation.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/15.6.1396