Prevalence and genotypic distribution of TT virus in Athens, Greece

The prevalence of TT virus (TTV) infection in various population groups from Athens, Greece, was assessed by the polymerase chain reaction (PCR) using two primer sets from distinct regions of the genome: the conventional set derived from the open reading frame‐1 (ORF‐1) and the new, highly sensitive...

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Bibliographic Details
Published in:Journal of medical virology 2001-10, Vol.65 (2), p.423-429
Main Authors: Katsoulidou, Antigoni, Paraskevis, Dimitrios, Anastassopoulou, Cleo G., Chryssou, Stella-Eugenie, Sypsa, Vassiliki, Boletis, John, Malliori, Meni, Karafoulidou, Anastasia, Tassopoulos, Nicholas C., Hatzakis, Angelos
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biological and medical sciences
DNA Primers
DNA Virus Infections - epidemiology
DNA, Viral - genetics
Female
Fundamental and applied biological sciences. Psychology
Genotype
genotypes
Greece - epidemiology
Humans
Male
Microbiology
Middle Aged
Molecular Sequence Data
nucleotide sequencing
Open Reading Frames
ORF-1 gene
ORF-2 gene
phylogenetic analysis
Phylogeny
Polymerase Chain Reaction
Torque teno virus - classification
Torque teno virus - genetics
TT virus
TTV DNA
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Summary:The prevalence of TT virus (TTV) infection in various population groups from Athens, Greece, was assessed by the polymerase chain reaction (PCR) using two primer sets from distinct regions of the genome: the conventional set derived from the open reading frame‐1 (ORF‐1) and the new, highly sensitive set targeting the region that includes the TATA signal localized upstream of ORF‐2. Based on both primer sets, TTV DNA was detected in 42/50 (84.0%) healthy individuals, 42/50 (84.0%) chronic hepatitis C patients, 31/39 (79.5%) acute non‐A–E hepatitis patients (group I), 14/16 (87.5%) renal failure patients with acute non‐A–E hepatitis (group II), 47/50 (94.0%) intravenous drug users (IVDU), 36/50 (72.0%) hemophiliacs, and 21/31 (67.7%) hemodialysis patients. The presence of TTV was not associated with any particular risk group, and no differences were observed in relation to demographic, biochemical and virological characteristics between TTV DNA‐positive and ‐negative patients. TTV did not seem to have a profound effect on the course of chronic C or acute non‐A–E hepatitis either. Phylogenetic analysis revealed that TTV strains circulating in the greater metropolitan area of Athens belong not only to the G1 and G2 genotypes that are encountered worldwide, but also to G3 and to G5 that are found mainly in Europe and Asia, respectively. Further studies will shed light on the role of this highly prevalent virus. J. Med. Virol. 65:423–429, 2001. © 2001 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.2052