Comparison of a clinic-based ELISA test kit with the immunofluorescence test for the assay of Ehrlichia canis antibodies in dogs

Israel Institute for Biological Research, Ness Ziona. The "gold standard" for the detection of antibodies to Ehrlichia canis, the cause of canine monocytic ehrlichiosis (CME), is the indirect immunofluorescence antibody (IFA) test. The IFA test however is generally available only in select...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2000-05, Vol.12 (3), p.240-244
Main Authors: Waner, T, Strenger, C, Keysary, A
Format: Article
Language:English
Subjects:
Animal bacterial diseases
Animals
Antibodies, Bacterial - blood
antibody detection
antibody formation
Bacterial diseases
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
disease diagnosis
Dog Diseases - diagnosis
Dog Diseases - microbiology
Dogs
Double-Blind Method
Ehrlichia - immunology
Ehrlichia - isolation & purification
Ehrlichia canis
Ehrlichiosis - diagnosis
Ehrlichiosis - microbiology
Ehrlichiosis - veterinary
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
experimental infections
fluorescent antibody technique
Fluorescent Antibody Technique, Indirect - veterinary
Fundamental and applied biological sciences. Psychology
immunoglobulin G
Immunoglobulin G - blood
Infectious diseases
Kinetics
Medical sciences
Microbiology
Reagent Kits, Diagnostic - veterinary
Sensitivity and Specificity
Statistics, Nonparametric
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Summary:Israel Institute for Biological Research, Ness Ziona. The "gold standard" for the detection of antibodies to Ehrlichia canis, the cause of canine monocytic ehrlichiosis (CME), is the indirect immunofluorescence antibody (IFA) test. The IFA test however is generally available only in selected laboratories and requires extensive equipment and trained personnel. A double-blind study was conducted to compare the ability of an in-clinic standardized enzyme-linked immunosorbent assay (ELISA) test kit to measure E. canis IgG antibodies in dogs compared with the standard IFA technique. A good correlation was found between the 2 techniques (r2 = 0.8793; P
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870001200307