Bonded dimethylacrylamide as a permanent coating for capillary electrophoresis

A method for coating capillaries for capillary electrophoresis with chemically bonded polydimethylacrylamide has been developed, and the properties of the capillaries have been evaluated. The coated capillaries provided high separation efficiency, 12·10 5 theoretical plates/m was obtained for cytoch...

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Bibliographic Details
Published in:Journal of Chromatography A 2001-07, Vol.924 (1), p.59-70
Main Authors: Wan, Hong, Öhman, Marcus, Blomberg, Lars G
Format: Article
Language:English
Subjects:
Acrylamides - chemistry
Adsorption
Amino acids
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Electrophoresis, Capillary - instrumentation
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Hydrogen-Ion Concentration
Osmosis
Other chromatographic methods
Peptide Fragments - isolation & purification
Peptides
Polydimethylacrylamide
Proteins
Reproducibility of Results
Surface Properties
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Summary:A method for coating capillaries for capillary electrophoresis with chemically bonded polydimethylacrylamide has been developed, and the properties of the capillaries have been evaluated. The coated capillaries provided high separation efficiency, 12·10 5 theoretical plates/m was obtained for cytochrome c. The electroosmotic flow at pH 8.0 was 10·10 −10 to 6·10 −10 m 2 V −1 s −1. The coated capillaries were quite stable at high pH. At least 150 runs could be done at pH 10 without appreciable performance deterioration. The excellent performance of the coated capillaries was illustrated by separation of basic proteins, acidic proteins, 9-fluorenylmethyl chloroformate-derivatized neurotransmitter amino acids, peptide reference mixtures and peptides digested from a bacteria protein.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)00715-4