A spirochete isolated from a case of severe virulent ovine foot disease is closely related to a Treponeme isolated from human periodontitis and bovine digital dermatitis

The isolation of spirochetes from severe ovine foot disease has been reported recently by our research group. In this study we describe the preliminary classification of this spirochete based on nucleotide sequence analysis of the PCR-amplified 16S rRNA gene. Phylogenetic analysis of this sequence i...

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Bibliographic Details
Published in:Veterinary microbiology 2000-06, Vol.74 (3), p.249-257
Main Authors: Collighan, Russell J, Naylor, Roger D, Martin, Patrick K, Cooley, Bill A, Buller, Nicky, Woodward, Martin J
Format: Article
Language:English
Subjects:
16S rRNA
Animal bacterial diseases
Animals
Bacterial diseases
Biological and medical sciences
Cloning, Molecular
Digital dermatitis
DNA Primers - chemistry
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
Electrophoresis, Agar Gel - veterinary
Foot Rot - classification
Foot Rot - microbiology
Foot-rot
Infectious diseases
Medical sciences
Microscopy, Electron - veterinary
Nucleic Acid Hybridization
Phylogeny
Plasmids - chemistry
Polymerase Chain Reaction - veterinary
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sheep
Sheep Diseases - classification
Sheep Diseases - microbiology
Sheep-bacteria
Treponema - chemistry
Treponema - classification
Treponema - genetics
Treponema - pathogenicity
Treponema vincentii
Treponemal Infections - microbiology
Treponemal Infections - veterinary
Virulence
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Summary:The isolation of spirochetes from severe ovine foot disease has been reported recently by our research group. In this study we describe the preliminary classification of this spirochete based on nucleotide sequence analysis of the PCR-amplified 16S rRNA gene. Phylogenetic analysis of this sequence in comparison with other previously reported 16S rRNA gene sequences showed that the spirochete belonged to the treponemal phylotype Treponema vincentii which has been associated with bovine digital dermatitis and human periodontal disease. Further work is required to define the common virulence determinants of these closely related treponemes in the aetiology of these tissue destructive diseases.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(00)00190-5