CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis)

: Cynomolgus monkeys were divided into two groups in terms of the reactivity of their lymphocytes with the FN18 monoclonal antibody, which is directed to the CD3 of rhesus monkeys. It was shown that 24 (12.2%) out of 196 monkeys did not have lymphocytes that reacted with the FN18, although T cells f...

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Bibliographic Details
Published in:Journal of medical primatology 2001-06, Vol.30 (3), p.141-147
Main Authors: Uda, Akihiko, Tanabayashi, Kiyoshi, Mukai, Ryozaburo, Yachi, Mayumi, Nam, Ki-Hoan, Yamada, Akio
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - genetics
Antibodies, Monoclonal - immunology
Base Sequence
CD3 Complex - genetics
Epitopes
Female
FN18
Macaca fascicularis - genetics
Male
Molecular Sequence Data
Polymorphism, Genetic
RT-PCR
sequence analysis
Signal Transduction
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Summary:: Cynomolgus monkeys were divided into two groups in terms of the reactivity of their lymphocytes with the FN18 monoclonal antibody, which is directed to the CD3 of rhesus monkeys. It was shown that 24 (12.2%) out of 196 monkeys did not have lymphocytes that reacted with the FN18, although T cells from those animals responded well to mitogenic stimulation. We have determined the nucleotide sequences of the CD3δ, CD3γ, and CD3ε chains and found that two amino acids of the CD3ε chain of the FN18 non‐reactive monkeys were different when compared with the FN18 reactive monkeys. Our results indicated that the CD3ε molecule of cynomolgus monkeys is polymorphic at the epitope level, which is recognized by the FN18 monoclonal antibody.
ISSN:0047-2565
1600-0684
DOI:10.1111/j.1600-0684.2001.tb00002.x