Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer

An amine-reactive caged rhodamine was synthesized and conjugated to aminodextran. The resulting tracer was injected into a single cell zebrafish embryo, and a portion of the tracer was photolyzed in a single cell after development. The resulting fluorescent cell was imaged by fluorescence microscopy...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2001-08, Vol.11 (16), p.2181-2183
Main Authors: Gee, Kyle R., Weinberg, Eric S., Kozlowski, David J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Dextrans - chemical synthesis
Dextrans - chemistry
Embryo, Nonmammalian
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Fundamental and applied biological sciences. Psychology
Microscopy, Fluorescence
Non mammalian vertebrate reproduction
Photochemistry
Rhodamines - chemical synthesis
Rhodamines - chemistry
Vertebrates: reproduction
Zebrafish
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Description
Summary:An amine-reactive caged rhodamine was synthesized and conjugated to aminodextran. The resulting tracer was injected into a single cell zebrafish embryo, and a portion of the tracer was photolyzed in a single cell after development. The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division. The caged tracer 5 was synthesized and used to follow cell division in a developing zebrafish embryo.
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(01)00421-8