Collision-Induced Dissociation of Mobility-Separated Ions Using an Orifice-Skimmer Cone at the Back of a Drift Tube

An ion mobility-mass spectrometry technique that incorporates a differentially pumped orifice-skimmer cone (OSC) region at the exit of the drift tube has been developed. The OSC region is similar in design to those used in electrospray ionization sources and offers improvements in ion transmission (...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-08, Vol.73 (15), p.3549-3555
Main Authors: Lee, Young Jin, Hoaglund-Hyzer, Cherokee S, Taraszka, John A, Zientara, Gina A, Counterman, Anne E, Clemmer, David E
Format: Article
Language:English
Subjects:
Analytical chemistry
Angiotensin II - analysis
Biological and medical sciences
Chemistry
Equipment Design
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Gases
Ions
Ions - analysis
Mass Spectrometry - instrumentation
Methods
Molecular and cellular biology
Scientific imaging
Spectrometric and optical methods
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Summary:An ion mobility-mass spectrometry technique that incorporates a differentially pumped orifice-skimmer cone (OSC) region at the exit of the drift tube has been developed. The OSC region is similar in design to those used in electrospray ionization sources and offers improvements in ion transmission (by factors of ∼5−10 compared with previous designs) and the ability to induce fragmentation of mobility-separated ions. The separation of ions prior to dissociation at the skimmer cone allows the origin of fragment ions to be examined. Here, we describe the experimental design and demonstrate the approach by examining fragment ions that are common to multiple charge states and different gas-phase ion conformations of electrosprayed angiotensin II and [Sar1, Val5, Ala8] angiotensin II peptides.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac010295e