Absorption of 2-hydroxy-4-(methylthio)butanoic acid by isolated sheep ruminal and omasal epithelia

Alimet (Novus Inter., Inc., St. Louis, MO) feed supplement (an 88% aqueous solution of 2-hydroxy-4-(methylthio) butanoic acid; HMB) is a source of L-Met commonly used in nonruminants and ruminants. The absorption of HMB across ovine omasal and ruminal epithelia was evaluated in this study. Ruminal a...

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Bibliographic Details
Published in:Journal of animal science 2000-04, Vol.78 (4), p.1078-1083
Main Authors: McCollum, M.Q, Vazquez-Anon, M, Dibner, J.J, Webb, K.E. Jr
Format: Article
Language:English
Subjects:
(s)-2-hydroxy-acid oxidase
active transport
alcohol oxidoreductases
Animal productions
Animals
Biological and medical sciences
d-2-hydroxy-acid dehydrogenase
enzyme activity
Enzymes
epithelium
Fundamental and applied biological sciences. Psychology
intestinal absorption
Intestinal Absorption - physiology
Intestinal Mucosa - metabolism
Kidney - metabolism
Kidneys
lambs
Liver
Liver - metabolism
methionine
Methionine - analogs & derivatives
Methionine - pharmacokinetics
methionine hydroxy analog
omasum
Omasum - metabolism
Rumen - metabolism
rumen epithelium
Sheep - physiology
Terrestrial animal productions
tissue culture
Vertebrates
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Summary:Alimet (Novus Inter., Inc., St. Louis, MO) feed supplement (an 88% aqueous solution of 2-hydroxy-4-(methylthio) butanoic acid; HMB) is a source of L-Met commonly used in nonruminants and ruminants. The absorption of HMB across ovine omasal and ruminal epithelia was evaluated in this study. Ruminal and omasal epithelia were collected from eight lambs (BW = 67.6 kg +/- 9.1) and mounted in parabiotic chambers that were repeatedly sampled throughout a 60-min incubation. The appearance of HMB (using DL-[5-(14)C]-HMB as a radiolabeled marker) in serosal buffers increased quadratically (P < .004) with time in both tissues. More (P < .001) HMB appeared in the serosal buffers with omasal than with ruminal epithelia. Both tissues responded similarly, and, after 60 min of incubation, the accumulation of HMB within the tissues increased linearly (P < .001) as substrate concentration (.375, .75, 1.5, 3.0, 6.0, and 12.0 mM) increased in mucosal buffers. As the concentration of HMB in the mucosal buffers increased, there was a quadratic (P
ISSN:0021-8812
1525-3163
0021-8812
DOI:10.2527/2000.7841078x