Oral Testosterone Administration Detected by Testosterone Glucuronidation Measured in Blood Spots Dried on Filter Paper

Blood sampling is not a common practice for sports drug testing. Our aim was to investigate whether dried blood spots on filter paper could be an alternative to plasma samples for monitoring steroid profiles in dope testing. We collected dried blood spots and plasma from six healthy Caucasian subjec...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2000-04, Vol.46 (4), p.515-522
Main Authors: Peng, Shi-Hua, Segura, Jordi, Farre, Magi, de la Torre, Xavier
Format: Article
Language:English
Subjects:
Administration, Oral
Adult
Biological and medical sciences
Biomarkers - blood
Blood Specimen Collection
Drug intoxications. Doping
Drug Stability
Gas Chromatography-Mass Spectrometry
Humans
Male
Medical sciences
Pharmacology. Drug treatments
Sensitivity and Specificity
Substance Abuse Detection - methods
Testosterone - administration & dosage
Testosterone - analogs & derivatives
Testosterone - blood
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Summary:Blood sampling is not a common practice for sports drug testing. Our aim was to investigate whether dried blood spots on filter paper could be an alternative to plasma samples for monitoring steroid profiles in dope testing. We collected dried blood spots and plasma from six healthy Caucasian subjects after an oral 120-mg dose of testosterone undecanoate (TU). Nonconjugated testosterone, testosterone glucuronide (TG), androsterone glucuronide (AG), and etiocholanolone glucuronide (EtG) were measured by gas chromatography-mass spectrometry in both matrices. 17alpha-Hydroxyprogesterone (17alphaOHP) and luteinizing hormone (LH) also were measured in the plasma samples. For comparison, similar measurements were done on samples obtained from the same subjects given 25 mg of testosterone propionate (TP) plus 110 mg of testosterone enanthate (TE) intramuscularly after a wash-out period. After oral TU intake, TG, AG, and EtG increased sharply, whereas nonconjugated testosterone did not change significantly. Results on dried blood spots correlated well with those on plasma. The TG/testosterone ratio in blood or plasma was verified to be a sensitive and specific marker (significantly increased for up to 8 h after intake; P
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/46.4.515