Microautoradiographic localisation of a glucosinolate precursor to specific cells in Brassica napus L. embryos indicates a separate transport pathway into myrosin cells

The in-situ localisation of a desulpho-glucosinolate precursor has been studied by microautoradiography of cryo-sections from immature seeds and pods of the high-glucosinolate Brassica napus L. cv. Argentine collected 23 days after pollination. After feeding with the tritium-labelled glucosinolate p...

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Bibliographic Details
Published in:Planta 2001-06, Vol.213 (2), p.207-213
Main Authors: Thangstad, Ole Petter, Bones, Atle Magnar, Holtan, Synnøve, Moen, Lene, Rossiter, John Trevor
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Agronomy. Soil science and plant productions
Ascorbic Acid - pharmacology
Autoradiography
Biological and medical sciences
Biosynthesis
Brassica napus
Brassica napus - cytology
Brassica napus - embryology
Brassica napus - growth & development
Brassica napus - metabolism
Carbon Radioisotopes
Cotyledons
Economic plant physiology
Embryonic stem cells
Embryos
freeze drying
Fundamental and applied biological sciences. Psychology
Glucosinolates
Glucosinolates - metabolism
Glycoside Hydrolases - metabolism
Metabolism
Metabolism. Physicochemical requirements
nitrogen
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
Plant physiology and development
Plant Structures - cytology
Plant Structures - embryology
Plant Structures - growth & development
Plants
pods
pollination
Seed pods
Seedlings
seeds
Stem cells
Sulfur
thioglucosidase
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Summary:The in-situ localisation of a desulpho-glucosinolate precursor has been studied by microautoradiography of cryo-sections from immature seeds and pods of the high-glucosinolate Brassica napus L. cv. Argentine collected 23 days after pollination. After feeding with the tritium-labelled glucosinolate precursor [4,5-³H](β-D-glucopyranosyl)-4-pentenethiohydroxamic acid, embryo radicles, cotyledons and pod-wall were frozen in liquid nitrogen. Cryotome sections were freeze-dried and coated with nuclear emulsion autoradiographic film. A distinct pattern of radioactivity derived from the glucosinolate precursor was found in specific cells in both radicle and cotyledons. In contrast, the labelling in pod walls was not cell specific, but general at the inner side of the pod wall. The results show that the glucosinolate/desulphoglucosinolate was localised in specific cells, in a pattern resembling that of myrosin cells known to contain myrosinase (EC 3.2.3.1). In addition [4,5-³H](β-D-glucopyranosyl)-4-pentenethiohydroxamic acid was fed to immature seeds and pods of B. napus and a quantitative incorporation into 2-hydroxy-3-butenylglucosinolate and 3-butenylglucosinolate was observed. When [4,5-³H](β-D-glucopyranosyl)-4-pentenethiohydroxamic acid was fed to 4-day-old seedlings the label was taken up by all tissues. We propose a model in which glucosinolate/desulphoglucosinolates are transported to myrosin cells to participate in the myrosinase-glucosinolate multifunctional defence system.
ISSN:0032-0935
1432-2048
DOI:10.1007/s004250000491