Transport mechanism and metabolism of olive oil hydroxytyrosol in Caco-2 cells

3,4-Dihydroxyphenylethanol (hydroxytyrosol; DPE) is the major phenolic antioxidant present in extra virgin olive oil, either in a free or esterified form. Despite its relevant biological effects, no data are available on its bioavailability and metabolism. The aim of the present study is to examine...

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Bibliographic Details
Published in:FEBS letters 2000-03, Vol.470 (3), p.341-344
Main Authors: Manna, Caterina, Galletti, Patrizia, Maisto, Giovanna, Cucciolla, Valeria, D’Angelo, Stefania, Zappia, Vincenzo
Format: Article
Language:English
Subjects:
Antioxidant
Antioxidants - metabolism
Biological Availability
Caco-2 cell
Caco-2 Cells
Catechol O-Methyltransferase - metabolism
Cell Differentiation
Cell Membrane Permeability
Chromatography, High Pressure Liquid
COMT, catechol-O-methyltransferase
Diffusion
DPE, hydroxytyrosol (3,4-dihydroxyphenylethanol)
Enterocytes - cytology
Enterocytes - metabolism
HMPE, homovanillic alcohol (3-hydroxy-4-methoxy phenylethanol)
Humans
Hydroxytyrosol
Kinetics
L-DOPA, 3,4-dihydroxy phenylalanine
Methylation
Olive Oil
P app, apparent permeability
PC, pyrocatechol (1,2-dihydroxybenzene)
PE, tyrosol (4-hydroxyphenylethanol)
Phenylethyl Alcohol - analogs & derivatives
Phenylethyl Alcohol - metabolism
Plant Oils - chemistry
Plant Oils - metabolism
Polyphenol
ROS, reactive oxygen species
Transport
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Summary:3,4-Dihydroxyphenylethanol (hydroxytyrosol; DPE) is the major phenolic antioxidant present in extra virgin olive oil, either in a free or esterified form. Despite its relevant biological effects, no data are available on its bioavailability and metabolism. The aim of the present study is to examine the molecular mechanism of DPE intestinal transport, using differentiated Caco-2 cell monolayers as the model system. The kinetic data demonstrate that [ 14C]DPE transport occurs via a passive diffusion mechanism and is bidirectional; the calculated apparent permeability coefficient indicates that the molecule is quantitatively absorbed at the intestinal level. The only labelled DPE metabolite detectable in the culture medium by HPLC (10% conversion) is 3-hydroxy-4-methoxyphenylethanol, the product of catechol- O-methyltransferase; when DPE is assayed in vitro with the purified enzyme a K m value of 40 μM has been calculated.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(00)01350-8