CLONING, SEQUENCING AND EXPRESSION OF A cDNA ENCODING THE MOUSE L35a RIBOSOMAL PROTEIN DURING DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA (MEL) CELLS

In a previous study we reported that ribosomal protein S5 gene is suppressed in differentiating and not in proliferating or apoptotic murine erythroleukaemia (MEL) cells (Vizirianakis et al., 1999). We wish to report here the isolation, characterisation and expression of the full length cDNA for ano...

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Bibliographic Details
Published in:Cell biology international 2001-07, Vol.25 (7), p.629-634
Main Authors: Pappas, I.S., Vizirianakis, I.S., Tsiftsoglou, A.S.
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Adenosine - pharmacology
Amino Acid Sequence
Animals
Base Sequence
Cell Differentiation - drug effects
Cloning, Molecular
differentiation
Dimethyl Sulfoxide - pharmacology
dimethylsulfoxide
DNA, Complementary
expression
L35a ribosomal protein
Leukemia, Erythroblastic, Acute - genetics
Leukemia, Erythroblastic, Acute - metabolism
Mice
Molecular Sequence Data
murine erythroleukemia cells
N6-methyladenosine
Pyridines - pharmacology
Ribosomal Proteins - genetics
Ribosomal Proteins - metabolism
RNA, Neoplasm - biosynthesis
Transcription, Genetic
Tumor Cells, Cultured
Urea - analogs & derivatives
Urea - pharmacology
ureido-derivative of pyridine-4
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Summary:In a previous study we reported that ribosomal protein S5 gene is suppressed in differentiating and not in proliferating or apoptotic murine erythroleukaemia (MEL) cells (Vizirianakis et al., 1999). We wish to report here the isolation, characterisation and expression of the full length cDNA for another ribosomal protein, the L35a (rpL35a), in MEL cells. This cDNA shares significant structural homology in both DNA and protein levels to genes encoding the rat and human L35a ribosomal proteins. Northern blot hybridisation analysis has shown that the steady-state level of rpL35a mRNA is progressively reduced during differentiation of MEL cells along the erythrocytic maturation pathway induced by DMSO or UDP-4, two structurally unrelated inducers of differentiation. However, in cells where differentiation was inhibited by N6-methyladenosine, the level of rpL35a RNA transcripts was not affected. In addition, rpL35a gene expression was not altered in apoptotic MEL cells. Furthermore, the suppression of L35a gene was not correlated to any change in DNA methylation at CCGG sites located at the rpL35a gene locus in undifferentiated and differentiated MEL cells, as we observed for the rpS5 gene. Overall, these data suggest that the expression of ribosomal genes, the L35a of 60S ribosomal subunit and the S5 of 40S ribosomal subunit, are regulated by a common mechanism in differentiating MEL cells, leading to the observed decrease in ribosomal function.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.2000.0695