New Insights into the Regulation of Cathepsin K Gene Expression by Osteoprotegerin Ligand

Cathepsin K plays a key role in bone resorption. We provide the first evidence that osteoprotegerin ligand (OPGL), a critical pro-resorptive cytokine, acutely stimulates the expression of cathepsin K in osteoclasts. We used in situ RT-PCR and real time quantitative RT-PCR to analyze cathepsin K gene...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-07, Vol.285 (2), p.335-339
Main Authors: Corisdeo, Susanne, Gyda, Michael, Zaidi, Mone, Moonga, Baljit S., Troen, Bruce R.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Bone Marrow Cells - cytology
Calcitriol - pharmacology
Carrier Proteins - pharmacology
Cathepsin K
Cathepsins - genetics
Cell Culture Techniques - methods
Cell Nucleus - physiology
Cells, Cultured
Cytokines - pharmacology
Femur
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Enzymologic - physiology
Kinetics
macrophage colony-stimulating factor
Macrophage Colony-Stimulating Factor - pharmacology
Membrane Glycoproteins - pharmacology
osteoclast
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - enzymology
osteoprotegerin
osteoprotegerin ligand
RANK Ligand
rat
Rats
Rats, Wistar
Recombinant Proteins - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Tibia
Time Factors
Transcription, Genetic - drug effects
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Summary:Cathepsin K plays a key role in bone resorption. We provide the first evidence that osteoprotegerin ligand (OPGL), a critical pro-resorptive cytokine, acutely stimulates the expression of cathepsin K in osteoclasts. We used in situ RT-PCR and real time quantitative RT-PCR to analyze cathepsin K gene expression. OPGL enhanced cathepsin K mRNA levels in mature osteoclasts isolated from rat neonatal long bones. OPGL together with macrophage colony-stimulating factor (M-CSF) also stimulated cathepsin K gene expression in monocytic cells and multinucleate osteoclasts in bone marrow cultures. Real time quantitative RT-PCR demonstrated high levels of cathepsin K mRNA in bone marrow cultures, paralleling the degree of osteoclastogenesis. We therefore suggest that OPGL enhances bone resorption, at least in part, by inducing cathepsin K gene expression.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5127