A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system

The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed...

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Bibliographic Details
Published in:Journal of neuroimmunology 2001-06, Vol.116 (2), p.188-195
Main Authors: Polfliet, Machteld M.J, Goede, Peter H, van Kesteren-Hendrikx, Esther M.L, van Rooijen, Nico, Dijkstra, Christine D, van den Berg, Timo K
Format: Article
Language:English
Subjects:
Analgesics, Non-Narcotic - pharmacology
Animals
Blood-Brain Barrier - immunology
Cell Count
Central Nervous System - blood supply
Central Nervous System - cytology
Central Nervous System - immunology
Clodronic Acid - pharmacology
Dichloromethylene-diphosphonate (Cl 2MDP)
ED2
Kinetics
Liposomes
Liposomes - pharmacology
Macrophages - cytology
Male
Meningeal macrophages
Meninges - blood supply
Meninges - cytology
Meninges - immunology
Perivascular macrophages
Rats
Rats, Wistar
Selective depletion
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Summary:The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed a strategy employing a single intraventricular injection of clodronate liposomes. This resulted in a complete depletion of the PVM and MM. Clodronate liposomes did not deplete the microglial cells. In other parts of the body, a temporal and mild depletion effect was observed, which was restored within 1 week. Detailed analysis of the elimination and repopulation kinetics of the PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion. This selective depletion method of the PVM and MM will enable us to get direct insight in their functions during normal and pathologic conditions of the CNS.
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(01)00282-X