Characterization of four cell lines derived from a human malignant fibrous histiocytoma of the maxillary sinus

We have established four cell lines from a human malignant fibrous histiocytoma. Each cell line had human aneuploid karyotype and DNA aneuploidy. Cells in all lines expressed CD13, CD68 and vimentin but lacked CD11, CD14, CD15, CD16, CD45, HLA class II and other mesenchymal and epithelial markers su...

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Bibliographic Details
Published in:Oral oncology 2001-09, Vol.37 (6), p.527-536
Main Authors: Mori, A, Tagawa, T, Kamei, T, Murata, T, Inui, M, Ohse, S
Format: Article
Language:English
Subjects:
3',5'-Cyclic-AMP Phosphodiesterases - metabolism
Aneuploidy
Animals
Antigens, CD - analysis
Antigens, Differentiation, Myelomonocytic - analysis
Biological and medical sciences
Biomarkers, Tumor - analysis
CD13 Antigens - analysis
Cyclic Nucleotide Phosphodiesterases, Type 3
Cytokine
Female
Flow Cytometry
Granulocyte Colony-Stimulating Factor - secretion
Histiocytoma, Benign Fibrous - genetics
Histiocytoma, Benign Fibrous - metabolism
Histiocytoma, Benign Fibrous - pathology
Histogenesis
Human malignant fibrous histiocytoma cell lines
Humans
Immunohistochemistry
Interleukin-1 - secretion
Interleukin-6 - secretion
Karyotyping
Maxillary sinus
Maxillary Sinus Neoplasms - genetics
Maxillary Sinus Neoplasms - metabolism
Maxillary Sinus Neoplasms - pathology
Medical sciences
Mesoderm
Mice
Mice, Nude
Middle Aged
Neoplasm Transplantation
Otorhinolaryngology. Stomatology
Platelet-Derived Growth Factor - secretion
Primitive mesenchymal cells
Procollagen-Proline Dioxygenase - metabolism
Tumor Cells, Cultured - metabolism
Tumor Cells, Cultured - pathology
Tumor Necrosis Factor-alpha - secretion
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
Vimentin - analysis
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Summary:We have established four cell lines from a human malignant fibrous histiocytoma. Each cell line had human aneuploid karyotype and DNA aneuploidy. Cells in all lines expressed CD13, CD68 and vimentin but lacked CD11, CD14, CD15, CD16, CD45, HLA class II and other mesenchymal and epithelial markers such as desmin, α-smooth muscle, myoglobin, S-100 protein, and cytokeratin. None of the cells expressed surface IgG or C3 receptor, nor did any of them phagocytose latex particles. The cells reacted with an antibody for prolyl-4-hydroxylase, but no collagen (types I, II, III, or IV) was detected in any of the cell lines. The homogenates of all cell lines had cyclic nucleotide phosphodiesterase 3 activity. Two cell lines produced granulocyte colony-stimulating factor (G-CSF), interleukin (IL)-1α, IL-6 and tumor necrosis factor α, another line produced G-CSF, IL-1α, IL-6 and platelet-derived growth factor (PDGF)-AB, and the remaining cell line produced only PDGF-AB. None of the cells produced transforming growth factor-α. The results indicated that the cell lines were immunophenotypically similar to primitive mesenchymal cells.
ISSN:1368-8375
1879-0593
DOI:10.1016/S1368-8375(01)00004-5