Genetic diversity of the Campylobacter genes coding immunodominant proteins

Abstract Three Campylobacter jejuni 72Dz/92 genes (cjaA (ompH1), cjaC (hisJ) and cjaD (omp18)) encoding immunodominant proteins are considered to be potential chicken vaccine candidates. The presence and conservation of cjaA, cjaC and cjaD genes among different Campylobacter clinical isolates were d...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-04, Vol.185 (1), p.43-49
Main Authors: Pawelec, Dariusz P., Korsak, Dorota, Wyszyńska, Agnieszka K., Rożynek, Elżbieta, Popowski, Janusz, Jagusztyn-Krynicka, Elżbieta K.
Format: Article
Language:English
Subjects:
ATP-Binding Cassette Transporters - genetics
Bacterial Outer Membrane Proteins - genetics
Bacterial Proteins
Base Sequence
Campylobacter
Campylobacter - classification
Campylobacter - genetics
Campylobacter - growth & development
Campylobacter - isolation & purification
Campylobacter coli - classification
Campylobacter coli - genetics
Campylobacter coli - growth & development
Campylobacter coli - isolation & purification
Campylobacter Infections - microbiology
Campylobacter jejuni - classification
Campylobacter jejuni - genetics
Campylobacter jejuni - growth & development
Campylobacter jejuni - isolation & purification
Campylobacter spp
Child
Clinical isolates
Codons
Conservation of the gene sequences
Conserved Sequence
Diarrhea - microbiology
Divergence
DNA, Bacterial - analysis
Gene sequencing
Genes
Genetic diversity
Genetic Variation - genetics
Genomes
Homology
Humans
Hybridization
Immunoblotting
Immunodominant Epitopes - genetics
Immunodominant protein
Infant, Newborn
Lipoproteins - genetics
Microbiology
MLST
Nucleotide sequence
Nucleotides
Outer membrane proteins
Pathogens
Polymerase Chain Reaction - methods
Polymorphism
Poultry
Proteins
Vaccines
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Summary:Abstract Three Campylobacter jejuni 72Dz/92 genes (cjaA (ompH1), cjaC (hisJ) and cjaD (omp18)) encoding immunodominant proteins are considered to be potential chicken vaccine candidates. The presence and conservation of cjaA, cjaC and cjaD genes among different Campylobacter clinical isolates were determined. The genes were detected in thirty Campylobacter strains using hybridization as well as Western blot analysis. However, PCR products of the predicted size were amplified only from ten out of thirty examined strains regardless of the employed primer pair. The nucleotide sequence of the C. jejuni 72Dz/92 genes was compared with the nucleotide sequences of their homologs cloned from other Campylobacter strains as well as with the whole genome sequence of C. jejuni NCTC 11168. The examined sequences revealed 0 to 16% divergence. Strain-dependent levels of divergence were observed. The polymorphism detected in cjaC was mainly within the 5′ region of the gene, while the nucleotide substitutions in cjaA and cjaD are distributed uniformly along the whole genes. Most of the observed nucleotide substitutions occurred at the third base of the codons. This observation is consistent with the results of Western blot experiments.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09038.x