Neutrophil CD11b Expression and Circulating Interleukin-8 as Diagnostic Markers for Early-Onset Neonatal Sepsis

To assess neutrophil CD11b and circulating interleukin 8 (IL-8) as markers of early-onset infection in neonates. The study comprised 39 neonates, with a gestational age of 29 to 41 weeks, suspected of infection within 48 hours of life. Neutrophil surface expression of CD11b was quantified with flow...

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Bibliographic Details
Published in:Pediatrics (Evanston) 2001-07, Vol.108 (1), p.e12-e12
Main Authors: Nupponen, Irmeli, Andersson, Sture, Jarvenpaa, Anna-Liisa, Kautiainen, Hannu, Repo, Heikki
Format: Article
Language:English
Subjects:
Biomarkers - blood
CD11 Antigens - blood
Humans
Infant, Newborn
Interleukin-8 - blood
Neutrophils - immunology
Pediatrics
Predictive Value of Tests
Prospective Studies
Sensitivity and Specificity
Sepsis
Sepsis - diagnosis
Sepsis - immunology
Time Factors
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Summary:To assess neutrophil CD11b and circulating interleukin 8 (IL-8) as markers of early-onset infection in neonates. The study comprised 39 neonates, with a gestational age of 29 to 41 weeks, suspected of infection within 48 hours of life. Neutrophil surface expression of CD11b was quantified with flow cytometry and plasma IL-8 with an enzyme-linked immunosorbent assay. Both data were available from 35 of 39 neonates. Serum C-reactive protein was determined at initial evaluation and, later, on the basis of the clinical picture. Neonates were allocated retrospectively into 2 groups. In the sepsis group (N = 22), 4 had culture-proven sepsis, and 14 had an antenatal risk factor for infection. In the possible-infection group (N = 13), each neonate had a noninfective disorder, but co-occurring infection remained a possibility. Twelve healthy term infants served as controls. CD11b expression and IL-8 levels both increased in order of sepsis > possible infection > healthy. Sensitivity and specificity by the CD11b test for sepsis were equal, at 1.00, and those by the IL-8 test 0.91 and 1.00, respectively; 6 (17.1%) of the 35 neonates had CD11b and IL-8 below cutoff levels. Measuring neutrophil CD11b expression and circulating IL-8 provides a means to identify early-onset neonatal sepsis. The findings may be helpful in planning strategies to safely reduce the use of antimicrobials in neonates.
ISSN:0031-4005
1098-4275
DOI:10.1542/peds.108.1.e12