Spatial, temporal and subcellular localization of islet-brain 1 (IB1), a homologue of JIP-1, in mouse brain

Islet‐brain 1 (IB1) was recently identified as a DNA‐binding protein of the GLUT2 gene promoter. The mouse IB1 is the rat and human homologue of the Jun‐interacting protein 1 (JIP‐1) which has been recognized as a key player in the regulation of c‐Jun amino‐terminal kinase (JNK) mitogen‐activated pr...

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Bibliographic Details
Published in:The European journal of neuroscience 2000-02, Vol.12 (2), p.621-632
Main Authors: Pellet, Jean-Baptiste, Haefliger, Jaques-Antoine, Staple, Julia K., Widmann, Christian, Welker, Egbert, Hirling, Harald, Bonny, Christophe, Nicod, Pascal, Catsicas, Stefan, Waeber, Gérard, Riederer, Beat M.
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Animals
Apoptosis
Brain - embryology
Brain - growth & development
Brain Chemistry
brain cytoskeleton
Carrier Proteins - chemistry
Cell death
Cells, Cultured
Cerebral Cortex - cytology
Cerebral Cortex - metabolism
Eye Proteins - analysis
Fetal Proteins - analysis
Gene Expression Regulation, Developmental
IB1
In Situ Hybridization
JIP-1
JNK
JNK Mitogen-Activated Protein Kinases
Kinases
Localization
MAP Kinase Signaling System
MAPK
Mice
Mitogen-Activated Protein Kinases - physiology
Nerve Tissue Proteins - analysis
Nuclear Proteins - analysis
Nuclear Proteins - biosynthesis
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Organ Specificity
Protein Isoforms - analysis
Protein Isoforms - biosynthesis
Protein Isoforms - chemistry
Protein Isoforms - genetics
Proteins
Rats
Rats, Wistar
Retina - chemistry
Retina - growth & development
Reverse Transcriptase Polymerase Chain Reaction
Signal transduction
Species Specificity
Subcellular Fractions - chemistry
Telencephalon - cytology
Telencephalon - metabolism
Trans-Activators - analysis
Trans-Activators - biosynthesis
Trans-Activators - chemistry
Trans-Activators - genetics
Transcription factors
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Summary:Islet‐brain 1 (IB1) was recently identified as a DNA‐binding protein of the GLUT2 gene promoter. The mouse IB1 is the rat and human homologue of the Jun‐interacting protein 1 (JIP‐1) which has been recognized as a key player in the regulation of c‐Jun amino‐terminal kinase (JNK) mitogen‐activated protein kinase (MAPK) pathways. JIP‐1 is involved in the control of apoptosis and may play a role in brain development and aging. Here, IB1 was studied in adult and developing mouse brain tissue by in situ hybridization, Northern and Western blot analysis at cellular and subcellular levels, as well as by immunocytochemistry in brain sections and cell cultures. IB1 expression was localized in the synaptic regions of the olfactory bulb, retina, cerebral and cerebellar cortex and hippocampus in the adult mouse brain. IB1 was also detected in a restricted number of axons, as in the mossy fibres from dentate gyrus in the hippocampus, and was found in soma, dendrites and axons of cerebellar Purkinje cells. After birth, IB1 expression peaks at postnatal day 15. IB1 was located in axonal and dendritic growth cones in primary telencephalon cells. By biochemical and subcellular fractionation of neuronal cells, IB1 was detected both in the cytosolic and membrane fractions. Taken together with previous data, the restricted neuronal expression of IB1 in developing and adult brain and its prominent localization in synapses suggest that the protein may be critical for cell signalling in developing and mature nerve terminals.
ISSN:0953-816X
1460-9568
DOI:10.1046/j.1460-9568.2000.00945.x